Comparisons of the outcomes between two commercial embryo culture media: HTF vs. GI

Anbari, F.; Khalili, M. A.; Nabi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p64
Academic Journal
Introduction: Currently, there are many different culture media available in assisted reproduction program. The quality of culture media may affect in-vitro development of cleavage embryos. This study compared the in-vitro outcomes between two different embryo culture media using mouse embryo assay (MEA). Materials and Method: Female mice were super ovulated with IP injection of 10 IU of PMSG followed by 10IU of HCG. Zygotes were collected 22-24 h after HCG administration. The oviducts with ampulla were dissected and placed in the MOPS-hepes media. The cumuli from zygotes were denuded until they were cumulus free. This process was performed 4 times until to achieve 80 zygotes. Zygotes were divided in experimental (HTF) and control (Vitrolife) groups. On day 5, the rates of expanded blastocysts were estimated. A safe label was assigned with min of 80% expanded blastocysts. Results: 20% and 68.75 % of the zygotes in experimental groups were arrested in 2 and 8 cells, respectively. 6 zygotes (7.5%) reached to morula stage and only 3 zygotes reached to expanded blastocysts that it is not acceptable. However in control group, 88% of embryos reached to expanded blastocysts stage. The rest (12%) arrested in embryo with 4-8 blatomeres. Conclusion: MEA is a useful and sensitive assay for quality control of human IVF culture media. It was obvious that commercial Gl media was appropriate for the in-vitro development of mouse embryos.


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