Meiotic spindle integrity after IVM of oocytes retrieved from ovarian tissue of sheep

Mohsenzadeh, M.; Khalili, M.; Taheri, F.; Fesahat, F.; Tabibnejhad, N.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p61
Academic Journal
Introduction: One of the fertility preservation methods for cancer patients would be in-vitro maturation (IVM) of oocytes with subsequent freezing. For fertility preservation, partial or total ovarian tissue can be removed. Follicular aspiration in order to segregate immature oocytes from ovarian cortex could be considered as a new technique along with freezing ovarian cortex. The aim was to evaluate the maturity rate and meiotic spindle status of matured oocytes following IVM of oocytes retrieved from ovarian tissue of sheep. Materials and Methods: This is an ongoing study including 36 fresh ovarian tissues of sheep. 74 immature (GV) oocytes were retrieved following follicular aspiration from ovarian cortex. After oocytes retrieval, the immature oocytes were transferred into IVM medium (TCM 199) and incubated at 37°C/5% CO2. Oocyte maturity and integrity of meiotic spindle were checked using light microscopy and polescope, respectively. Results: 54 oocytes with dark cytoplasm/ sign of degeneration were denuded following aspiration. These oocytes were then discarded. Only 20 GV oocytes had bright and normal cytoplasm and cumulus cells. These oocytes underwent IVM process, and 20% of these matured oocytes showed normal meiotic spindle with sign of maturity. Conclusion: IVM technology can be advanced with assessment of meiotic spindle integrity, especially for fertility preservation purposes. More animal samples are needed to pinpoint the cause of oocytes degeneration in this study.


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