TITLE

The effects of lead on motility, viability and DNA denaturation of cauda epididymal spermatozoa of mouse

AUTHOR(S)
Fazelian, K.; Golshan-Iranpour, F.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p57
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Lead is one of the environmental contaminants. The aim of this study was to investigate the effects of lead on motility, viability and DNA denaturation of mouse spermatozoa. Materials and Methods: 24 male mice were considered as case group and 24 mice as control group. The case group was injected by one dose of 200 mg/kg of lead acetate and control group was injected only by distilled water. Each group (case and control groups) was divided into three subgroups and the members of each subgroup were killed after 1, 2 and 3 weeks. Motility, viability and DNA denaturation of caudal epididymal spermatozoa were examined in all groups. Data were analyzed using Mann-Witney U and Kruskal-Wallis tests. Results: Lead reduces the motility and viability of spermatozoa one week after the injection. These effects of lead were disappeared three weeks after the injection. In contrast to the effect of lead on motility and viability, it did not affect on DNA denaturation. Conclusion: Contamination with lead can reduce motility and viability of sperms but these effects will be disappeared by the time. Lead cannot create any changes on DNA denaturation of spermatozoa.
ACCESSION #
96841616

 

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