Sperm separation in the presence of ascorbic acid and albumin

Alahmoradi, M.; Khanbabaee, R.; Seyedalipour, B.; Shojaie, S.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p55
Academic Journal
Introduction: By placing the mouse spermatozoa in media containing certain concentrations of albumin and ascorbic acid, the medium effect on separation of spermatozoa containing X or Y chromosome, in IVF and embryo transfer was studied. Materials and Methods: 15 adult NMRI mice were randomly chosen and divided into three groups, control group and experimental groups. Initial evaluation of sperm samples, in the presence of concentrations of ascorbic acid and albumin was done. In this case study, The mentioned mouse spermatozoa which were separated & located in IVF dish, Near the same race mice oocytes by the injection of PMSG (Pregnant Mare Serum Gonadotrophin) and HCG (Human Chorionic Gonadatrophin), fetuses were transferred to the surrogate mother (experimental group). Results: The mean percentage of male infants born in the experimental group was significantly different from the control group (p<0.05). Of five surrogate mothers, which were pregnant with sperm separation in the presence of ascorbic acid (750 and 700 micromolar), and albumin concentrations, The amount of male achievement in the presence of 750 micromolars concentration of ascorbic acid was 75.4291%, 700 micromolars concentration of ascorbic acid was 76.2857%, and in the control group was 49.9%. Adding ascorbic acid (750 and 700 micromolar) and albumin resulted in fat peroxidation reduction of the spermatozoeid medium extracted from adult mouse. Conclusion: This method is suitable for sperm containing X and Y chromosomes separation and male sex selection in fertile couple.


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