Comparison of serum matrix metalloproteinase-9 (MMP-9) and NGAL/MMP-9 complex activity in polycystic ovary syndrome (PCOS) and control women

Amiry, I.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p53
Academic Journal
Introduction: Epidemiological studies estimates the prevalence of 6.5-8% for PCOS in women of reproductive age, using biochemical and/or clinical evidence, while ultrasound-based studies have reported a prevalence of 20% or more. The syndrome is associated with dyslipidemia, obesity, insulin resistance, hypertension, and metabolic syndrome. The remodeling of the ovary extracellular environment is dependent upon the breakdown of the follicular extracellular architecture by matrix metalloproteinase (MMP) enzymes such as MMP-9 ad Neutrophil Gelatinase-Associated Lipocalin (NGAL) complex with MMP-9. The current study investigates the role of MMP-9 and NGAL/MMP-9 complex activity in PCOS patients. Materials and Methods: In this case- control match-paired study, 40 patients with polycystic ovaries and 40 normally ovulating women were enrolled. Blood samples were collected and serum was separated. Circulating levels of FBS and lipid profile were measured using enzymatic methods, whereas ELISA kits were used to determine the concentration of LH, FSH, testosterone, esteradiol, and sex hormone binding globulin (SHBG) as well as serum MMP-9 , NGAL/MMP-9, and tissue inhibitor of matrix metal loproteinase-1 (TIMP-1). Additionally, the zymography technique was applied to obtain the enzymatic activity of MMP-9 and NGA/MMP-9 complex. Results: No significant difference was observed in FBS, lipid profiles, esteradiol and SHBG between groups while significantly higher levels of LH, LH/FSH ratio, and testosterone were found in PCOS patients compared to controls. Conclusion: The concentration of MMP-9, TIMP-1, and NGAL/MMP-9 complex did not differ between groups whereas patients with polycystic ovaries showed a significantly higher activity of MMP-9.


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