The effects of antioxidant supplement on human sperm after the freezing-thawing process

Farhmani, M.; Amiri, I.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p53
Academic Journal
Introduction: The aim of this study was to determine the effects of antioxidants Butylated Hydroxi Toluene and Glutathione on microscopic semen parameters, lipid peroxidation, the level of Reactive Oxygen Species and DNA fragmentation following freezing- thawing of human semen. Materials and Methods: Semen samples were obtained from sixty normospermic donors and each three semen were pooled. The pooled semen samples were processed with density gradient method using pure sperm. After processing, the pellet was divided to three parts. The first parts were freezing medium containing BHT (0.5 mM) and the second parts were diluted with Glutathione (5 mM) and the third parts were diluted in freezing medium with no antioxidant (control). Then all samples were frozen and stored in liquid nitrogen. After 3 weeks samples warmed for two min in a water bath (37*#176;C) for evaluation the effects of antioxidants on sperm. Results: Freezing extenders supplemented with 0.5 BHT and Glutathione (5mM) led to higher sperm motility and viability compared to the control (p<0.00). The addition of antioxidants decreased malondialdehyde ROS formation, MDH formation and DNA fragmentation compared to the controls. Conclusion: Our results showed that the addition of GSH and BHT to the freezing extender could be of partial and limited benefit in improving the function of frozen human spermatozoa.


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