TITLE

Comparison of follicular fluid and MEM in maturation and fertilization of immature mouse oocyte

AUTHOR(S)
Abbaszadeh, H.; Khoramgah, M.; Nourozian, M.; Sadeghi, Y.; Keramatinia, A.; Hoseini, A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p43
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. This method is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study has been designed to assess the effect of heated human follicular fluid (hHFF), which is similar to in vivo environment for oocyte, on the maturation and fertilization potential of mouse immature oocytes. Materials and Methods: Healthy female mice were sacrificed via cervical dislocation. Immature oocytes were placed in an incubator for 24 hours. Then, the stages of oocyte maturation were assessed by invert microscope and mature oocytes in each group were transferred to sperm-contained drops. After 24 hr, rate of two-cell embryos was recorded using invert microscope. Data was analyzed by Chi square test. Results: Maturation rate of oocytes in the second group (83.7%) was significantly higher than first (59.8%) and third (67.3%) groups (p<0.0005). The formation rate of two-cell embryo in the second group (83.1%) was higher than first (47.2%) and third (54/3%) groups (p<0.002 and p<0.01, respectively). Conclusion: It seems hHFF could improve in vitro maturation and fertility potential of immature oocytes and consequently the formation rate of two-cell embryos in mice.
ACCESSION #
96841583

 

Related Articles

  • Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Yi-Liang Miao; Kikuchi, Kazuhiro; Qing-Yuan Sun; Schatten, Heide // Human Reproduction Update;Sep2009, Vol. 15 Issue 5, p573 

    BACKGROUND: In humans, normal healthy children are regularly produced through fertilization of fresh oocytes with fresh spermatozoa. However, asynchrony between oocytes and spermatozoa, especially when aged oocytes are fertilized by fresh or senescent spermatozoa, will not only affect the rate...

  • Clinical benefit of metaphase I oocytes. Vanhoutte, Leen; De Sutter, Petra; Van Der Elst, Josiane; Dhont, Marc // Reproductive Biology & Endocrinology;2005, Vol. 3, p71 

    Background: We studied the benefit of using in vitro matured metaphase I (MI) oocytes for ICSI in patients with a maximum of 6 mature metaphase II (MII) oocytes at retrieval. Methods: In 2004, 187 ICSI cycles were selected in which maximum 6 MII oocytes and at least one MI oocyte were retrieved....

  • Lipofuscin bodies in human oocytes as an indicator of oocyte quality. Otsuki, Junko; Nagai, Yasushi; Chiba, Kazuyoshi // Journal of Assisted Reproduction & Genetics;Jul2007, Vol. 24 Issue 7, p263 

    Refractile bodies are one of the main morphological abnormalities that can be observed in the cytoplasm of human oocytes. In the present studies the characteristics of refractile bodies and the relationship between the size of these structures and developmental competence of the affected oocytes...

  • Binovular complexes after ovarian stimulation. A report of four cases. Asimakopoulos, B.; Kotanidis, L.; Nikolettos, N. // Hippokratia;2013, Vol. 17 Issue 2, p169 

    Background: Human ovary is known to contain polyovular follicles at birth but it is considered to be rare in adult ovaries. Cases: Four cases are presented where binovular complexes were found after ovarian stimulation. The age of women was 22 to 33 years and all of them had a good response to...

  • blastocyst. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p105 

    An encyclopedia entry for the term "blastocyst" is presented. It refers to the cell cluster which is formed through a fertilized ovum. This cell cluster eventually becomes an embryo.

  • Recurrent implantation failure: definition. Rinehart, John // Journal of Assisted Reproduction & Genetics;Jul2007, Vol. 24 Issue 7, p284 

    Purpose An attempt to define recurrent implantation failure. Methods Literature review. Results Numerous definitions of recurrent implantation failure exist. The complexity and redundancy of the process of implantation makes a single definition difficult to determine. Conclusion Each center must...

  • No Correlation Exists between Mean Oocyte Diameter and Fertilization or Embryo Quality.  // Fertility Weekly;2/22/2010, p9 

    The article presents a study which aims to further measure the effect of mean oocyte diameter (MOD) on fertilization and embryo quality in humans. Finding shows that the mean diameter of a mature oocyte does not seem to be associated to the occurrence of fertilization or to the growth quality of...

  • SELECTED ORAL COMMUNICATION SESSION, SESSION 49: EMBRYOLOGY – THE FERTILIZATION PROCESS, Tuesday 5 July 2011 17:00 – 18:00.  // Human Reproduction;Jan2011 Supplement 1, Vol. 26 Issue suppl_1, pi73 

    No abstract available.

  • Fewer Oocytes Retrieved with Highly Purified hMG than with rFSH.  // Fertility Weekly;7/14/2008, p4 

    The article reports on the study by Dutch researchers which reveals that hMG treatment results in more pregnancies and live births compared with rFSH. The study involved 629 patients who underwent GhRH agonist suppression in the long protocol while IVF-ICSI and embryo transfer were conducted. It...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics