Evaluating the level of reactive oxygen species in seminal fluid of men with recurrent pregnancy loss; does it have any role?

Moein, M. R.; Ghasemzadeh, J.; Talebi, A. R.; Halvaei, I.; Nabi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p42
Academic Journal
Introduction: It is estimated that about 50% of spontaneous abortion might have preventable causes. The main goal of this study was to compare concentration of ROS in seminal fluid between RPL and fertile groups. Materials and Methods: In this case control study we selected 28 men with history of RPL who were referred to our clinic and 27 fertile men who had normal sperm parameters and also had a child one year as case and control groups, respectively. Makler chamber and phase contrast microscopy at ×200 magnification was used for evaluation of sperm count, sperm motility and morphology. Evaluation of ROS was done by chemilumine scence assay. Independent sample t-test and Mann-Whitney U test were used to compare the data between groups. Results: There was insignificant difference between age of men in case and control groups (mean± SD: 31.7±4.8 and 34.4±5.4, respectively). Sperm count, motility and morphology were comparative in both groups with no significant differences. Although the ROS level showed the increasing trend in the case group compared to controls (median (min-max): 126.5(10-785) and 49.6(7-658), respectively) but the difference was insignificant. Conclusion: Our data showed that the ROS level may have increased level in RPL cases. More studies are needed to elucidate the probable role of ROS in etiology of RPL.


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