Does anti-oxidant therapy add any extra benefit to standard inguinal varicocelectomy in terms of DNA damage or sperm quality factor indices? A randomized study

Pourraand, G.; Movahedin, M.; Mehrsai, A.; Noori, M.; Dehghani, S.; Ahmadi, A.; Pourhosein, M.; Hoseini, M.; Ziloochi, M.; Heidari, F.; Beladi, L.; Alizadeh, F.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p40
Academic Journal
Introduction: Varicocele is the leading cause of male factor infertility, responsible for up to 40% and 80% of primary and secondary infertilities, respectively. Disturbance in oxidative stress, DNA damage and semen parameters has several possible factors that can also define varicocele impact on fertility. It was documented that varicocelectomy will improve semen analysis parameters and DNA damage in infertile patients with varicocele. We proposed to investigate the role of adding anti-oxidant to this therapy in terms of improving semen parameters and DNA damage. Materials and Methods: 100 patients enrolled in this study and were randomly divided into two groups (50 patients in each group). In Group 1, standard inguinal varicocelectomy and in group 2, standard inguinal varicocelectomy plus oral anti-oxidant therapy (oral L-Carnitine 250 mg three times a day) was performed for six months. For all patients, routine semen analysis plus DNA damage test of spermatozoa (by two methods of TUNEL and Protamine Damage Assay) were performed at baseline and three and six months post operatively. Results: In both groups, the improvement in semen analysis parameters and DNA damage was observed but there was not any statistically significant difference between the two groups in these parameters, although the slope of improvement in DNA damage was slightly better in Group 2 (that was not statistically significant). Conclusion: We observed that adding anti-oxidant therapy to standard inguinal varicocelectomy does not add any extra benefit in terms of semen analysis parameters to DNA damage.


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