Evaluation of sperm global DNA methylation and chromatin structure in infertile men with varicocele and fertile men

Bahreinian, M.; Tavalaee, M.; Nasr-Esfahani, M. H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p39
Academic Journal
Introduction: DNA methylation is an important event in the processes such as embryonic development, genomic imprinting, X-chromosome inactivation, and chromosome stability. Studies suggested stress oxidative in infertile men especially in individuals with varicocele is higher than fertile individuals. In this regard, a negative correlation between DNA methylation and stress oxidative in infertile men were reported. In addition sperm DNA damage could result from aberrant chromatin packaging during spermatogenesis, defective apoptosis and/ or excessive production of reactive oxygen species. We aimed to evaluation correlation between stress oxidative, DNA methylation and DNA damage in individual with varicocele. Materials and Methods: Semen sample from 44 individuals with varicocele II, III were collected. Sperm parameters (WHO criteria), protamine deficiency (CMA3 staining), stress oxidative cell (DCFH-DA staining), global methylation DNA (Immuno staining) and DNA damage (TUNEL assay) were evaluated. Results: Correlation analysis revealed that there is a negative significant correlation between DNA methylation and DNA fragmentation ,but not with degree of protamine deficiency and ROS production . Suggesting that in sperm with high DNA damage , percentage DNA methylation is low. Conclusion: DNA hypomethylation in individuals with varicocele prone spenn to DNA damage, independent of ROS production.


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