Study of Wnt3a protein concentration and expression of frizzled 10 (FZD10) and GSK3-β genes in testicular tissue of obstructive and non-obstructive azospermic men

Nazarian, H.; Ghaffari Novin, M.; Hosseini, S. J.; Mirfakhraie, R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p38
Academic Journal
Introduction: The Wnt/β-catenin signaling pathway controls many developmental processes during the entire lifetime where its disorders can cause a wide range of pathologic defects including cancer in both animals and humans. It has some key roles in reproductive processes. The aim of this study was to compare expression of FZD10 gene (the main receptor) and GSK3-β gene (the key regulator) of this signaling pathway in obstructive and non-obstructive azoospermic men. Materials and Methods: WNT3a protein concentration, expression level of FZD10 and GSK3-β genes were measured and compared between two groups of infertile men (with obstructive and nonobstructive azoospermia). Samples were obtained by testicular biopsy and screened for the most common mutations (84, 86 and 255) in the SRY region and CFTR before analyzing. FZD10 and GSK3-β gene expression was assessed quantitatively by real time-PCR. Results: The WNT3a protein concentration had no significant difference between the two test groups and controls. Expression level of FZD10 gene had no significant difference between two patient groups and control, however was slightly less in non-obstructive azoospermic men. Expression of GSK3-β was down-regulated in non-obstructive azoospermia compared with normal and obstructive azoospermia groups (p<0.05). Conclusion: Down-regulation of GSK3-β may cause to non-obstructive azoospermia in which the spermatogenesis is impalanced. Regulation and modification of GSK3-β gene expression by drugs could be used as a therapeutic solution.


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