TITLE

Comparison of Bax, BcI-2 and ErbB4 genes expression in vitrified and re-vitrified mouse blastocysts

AUTHOR(S)
Majidi Gharenaz, N.; Movahedin, M.; Mazaheri, Z.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p36
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Nowadays, using of vitrification to preserve of embryo is a routine technique. Re-vitrification, is applied in some situations like unprepared paitient's endometrium. In this study profiles of Bax, Bcl-2 and ErbB4 genes were evaluated in vitrified and re-vitrified mouse blastocysts. Materials and Methods: 5-8 cell embryos were collected from female mature mice, 60-62 h post hCG injection. The embryos were divided to three groups included: fresh, vitrified at blastocyst stage, vitrified (in 5-8 cells stage) and then re-vitrified at blastocyst stage. Vitrification was done using cryolock method according Kuwayama report. The relative quantification of Bax, Bcl-2 and ErbB4 genes was carried out by real time PCR. Total RNA was isolated from blastocyst embryos using QIAzol. RAN was transcribed to cDNA. Then real time PCR was performed. The data analyzed with ANOVA. Results: Our data showed that expression of Bax (pro apoptotic gene), Bcl-2 (anti apoptotic gene) had a significant difference (p<0.05) between re-vitrified and fresh embryos. Although; there was no significant difference between re-vitirified and vitrified embryos. Similar to pervious genes, ErbB4 gene expression had also a significant difference (p<0.05) between re-vitrified and fresh embryos. Re-vitrification down regulated the expression of ErbB4 (implanting gene). Although there was no significant difference between re-vitirified and vitrified embryos regarding genes expression. Conclusion: Based on our study, re-vitrification like vitrification could reduce expression of implanting gene and altered apoptotic genes expression compared to fresh embryo.
ACCESSION #
96841564

 

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