Effect of GDF-9 supplement on in vitro maturation of human GV oocytes: spindle visualization and ZP birefringence

Ashourzadeh, S.; Khalili, M. A.; Kalantar, S. M.; Aflatoonian, A.; Habibzadeh, V.; Nematollahi Mahani, S. N.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p35
Academic Journal
Introduction: GDF-9 is an oocyte-secreted GF which is critical for promotion of ovarian follicle growth in vitro and preovulatory cumulus expansion, in addition it is mitogenic factor for granulose and theca cells and anti-apoptotic in preantral follicles. So supplementation of GDF-9 in IVM medium may enhance embryo development and fetal viability. The aim was to investigate the effects of exogenous GDF9 during IVM of human GV oocytes retrieved from stimulated ICSI cycles, on oocyte maturation, fertilization and subsequent embryo development. Materials and Methods: Retrieved GV oocytes divided in 2 groups. In group I, oocytes cultured in commercial IVM media (SAGE) and group II, oocytes cultured in media that supplemented with 200 ng/ml exogenous GDF-9 (Sigma ) at 37°C in, 5% CO, with high humidity. Maturation was considered when they had the first polar body. Matured oocytes were screened for ZP birefringence and existing of miotic spindles with Polar Aide Microscope. After ICSI, normal fertilization and further cleavage was analyzed up to 4 cell embryo. Results: 140 GV oocytes were cultured in group I and 59 GV oocytes in group II. The overall maturation rate was 68.57% vs. 66.10% in groups I and II respectively. Although, there were not any significant differences between groups in terms of spindle visualization, ZP birefringence and the fertilization rates, but the rate of embryo formation was significantly higher in group II compared with group I (55.3% vs. 34.8%; p=0.02). Conclusion: Application of exogenous GDF9 during clinical IVM improved embryo development. It seems a promising approach for improving human IVM.


Related Articles

  • Polar Body Biopsy in the Diagnosis of Monogenic Diseases-The Birth of Three Healthy Children. Griesinger, Georg; Bündgen, Nana; Salmen, Diana; Schwinger, Eberhard; Gillessen-Kaesbach, Gabriele; Diedrich, Klaus // Deutsches Aerzteblatt International;8/14/2009, Vol. 106 Issue 33, p1 

    The article focuses on the use of the polar body analysis (PBA) in the diagnosis of monogenic diseases (MD) in Germany. For carriers of MD in Germany who want preconceptional diagnosis, PBA is the only way to contemplate pregnancy without any risk. Every PBA needs in vitro fertilization and...

  • Two cases of dizygotic triplet pregnancy following conventional in vitro fertilization. Furugori, Kazunori; Ando, Hisao; Ito, Tomomi; Mizutani, Shigehiko // Reproductive Medicine & Biology;Mar2003, Vol. 2 Issue 1, p41 

    The mechanism of monozygotic multifetal pregnancy and its association with assisted reproductive technology are uncertain. This report presents two cases of dizygotic triplet pregnancy after the transfer of three embryos in utero . The incidence of monozygotic twinning following assisted...

  • One-step collagenase method for zona pellucida removal in unfertilized eggs: easy and gentle method for large-scale preparation. Yamatoya, Kenji; Ito, Chizuru; Araki, Motoyuki; Furuse, Ryoji; Toshimori, Kiyotaka // Reproductive Medicine & Biology;Jun2011, Vol. 10 Issue 2, p97 

    Purpose: Zona pellucida (ZP)-free eggs are often used for studies such as evaluating the interaction of sperm-oolemma. To acquire ZP-free eggs, the most commonly used methods employ acidified Tyrode's solution, enzymatic digestion with a trypsin-like enzyme, or mechanical methods using...

  • Case Report: Pregnancy outcome following ICSI of oocytes with abnormal cytoplasm and zona pellucida. Paz, Gedalia; Amit, Ami; Yavetz, Haim // Human Reproduction;Vol. 19 Issue 3, p586 

    A couple with unexplained infertilility was referred for routine IVF and ICSI treatment. Ovulation was induced by the GnRH analogue protocol combined with HMG and HCG. Preparation of denuded oocytes revealed a major disorder of the zona pellucida and abnormal oocytes. During preparation of ova...

  • Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study. Mehranjani, Malek Soleimani; Noorafshan, Ali; Hamta, Ahmad; Momeni, Hamid Reza; Abnosi, Mohammad Hussein; Mahmoodi, Monireh; Anvari, Morteza; Hazaveh, Maryam // Iranian Journal of Reproductive Medicine;Winter2010, Vol. 8 Issue 1, p1 

    Background: Para-Nonylphenol (p-NP) is one of the environmental pollutants which cause reproductive system disorders. Objective: The effects of vitamin E on ovary structure during its development in rats treated with p-NP. Materials and Methods: 32 Wistar female rats after mating were divided...

  • New ART Method for Use with Cryopreserved Spermatozoa.  // Fertility Weekly;4/8/2002, p16 

    Researchers in Japan used the piezo electric actuator to develop a new assisted reproductive technology for improvement of the fertility rate of cryopreserved mouse spermatozoa. The cryopreservation of mouse spermatozoa is a widely used technique. Although partial zona pellucida dissection helps...

  • Examination of Risk of Ovulation.  // Fertility Weekly;4/8/2002, p18 

    Researchers in Japan used the piezo electric actuator to develop a new assisted reproductive technology for improvement of the fertility rate of cryopreserved mouse spermatozoa. The cryopreservation of mouse spermatozoa is a widely used technique. Although partial zona pellucida dissection helps...

  • Defective Sperm-Zona Pellucida Interactions Occur More Often in Oligozoospermic Men.  // Fertility Weekly;2/2/2004, p9 

    Researchers in Australia report that infertile men with oligozoospermia frequently have defective sperm-zona pellucida (ZP) interaction. Oligozoospermia is traditionally defined by a sperm concentration of less than 20 x 106/ml. The aim of this study by researchers De Yi Liu and H.W.G. Baker was...

  • Sequence Variations: High Degree of Variations in Zona Pellucida Genes of Infertile Women.  // Fertility Weekly;8/8/2005, p6 

    The article reports on a study related to the zona pellucida (ZP) genes of infertile women which revealed a high degree of sequence variation among genes. According to researchers in the U.S., 18 infertile women with total fertilization failure who's in-vitro fertilization (IVF) failed. The...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics