TITLE

Effect of GDF-9 supplement on in vitro maturation of human GV oocytes: spindle visualization and ZP birefringence

AUTHOR(S)
Ashourzadeh, S.; Khalili, M. A.; Kalantar, S. M.; Aflatoonian, A.; Habibzadeh, V.; Nematollahi Mahani, S. N.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p35
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: GDF-9 is an oocyte-secreted GF which is critical for promotion of ovarian follicle growth in vitro and preovulatory cumulus expansion, in addition it is mitogenic factor for granulose and theca cells and anti-apoptotic in preantral follicles. So supplementation of GDF-9 in IVM medium may enhance embryo development and fetal viability. The aim was to investigate the effects of exogenous GDF9 during IVM of human GV oocytes retrieved from stimulated ICSI cycles, on oocyte maturation, fertilization and subsequent embryo development. Materials and Methods: Retrieved GV oocytes divided in 2 groups. In group I, oocytes cultured in commercial IVM media (SAGE) and group II, oocytes cultured in media that supplemented with 200 ng/ml exogenous GDF-9 (Sigma ) at 37°C in, 5% CO, with high humidity. Maturation was considered when they had the first polar body. Matured oocytes were screened for ZP birefringence and existing of miotic spindles with Polar Aide Microscope. After ICSI, normal fertilization and further cleavage was analyzed up to 4 cell embryo. Results: 140 GV oocytes were cultured in group I and 59 GV oocytes in group II. The overall maturation rate was 68.57% vs. 66.10% in groups I and II respectively. Although, there were not any significant differences between groups in terms of spindle visualization, ZP birefringence and the fertilization rates, but the rate of embryo formation was significantly higher in group II compared with group I (55.3% vs. 34.8%; p=0.02). Conclusion: Application of exogenous GDF9 during clinical IVM improved embryo development. It seems a promising approach for improving human IVM.
ACCESSION #
96841563

 

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