The effects of estradiol and ECG on oocyte morphology and fertilization rate in mouse oocyte

Mohammadzadeh, M.; Shahbazi, M.; Haidari, K.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p35
Academic Journal
Introduction: Ovarian stimulators make some changes on ovarian follicle development and they crucial role in the field of reproductive biology. On the other hands, some oocyte features consist of Zona Pellucida and Perivitelline space (PVS) morphology have an impact on fertilization rate, embryo quality and implantation. Therefore our attempts have been made to delineate the effects of combined Estradiol and eCG on oocyte morphology and fertilization rate in here. Materials and Methods: Sixty female NMRI mice in the three groups intraperitoneally received normal saline, eCG (10IU) and also eCG (10IU) + Estradiol (E2) (1 μg) in same injection volumes respectively. After 48-hour, they received hCG (10IU) due to ovulation induction. The half of yielded oocytes designed for achieving IVF and another one for measuring ZP3 and PVS diameters. Results: There were significant differences in the mature oocytes, PVS diameter in the group which were only received eCG in comparison with others. Conclusion: It concluded that eCG alone is more effective on yielding more mature oocytes and PVS diameter rather than eCG+E2.


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