TITLE

Effect of vitrification on ultrastructure of cumulus cells of human mature oocyte

AUTHOR(S)
Taghizabet, N.; Khalili, M. A.; Talebi, A. R.; Anvari, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p34
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Cryopreservation of the intact cumulus oocyte complex is problematic because the optimal times for equilibration are likely different for the oocyte than its diminutive cumulus cells effects of cumulus cells on oocyte survival after freezing and thawing are controversial. The purpose of this study was to explore the effects of vitrification cryopreservation on ultrastructure of cumulus cells of human mature oocytes. Materials and Methods: The samples were divided into group A (cumulus cells of human mature oocytes without oocyte were vitrification) and group B (cumulus cells of human mature oocytes with oocyte were vitrified). Based on no significant differences in age, duration of infertility, infertile causes. Transmission electron microscopy was used to compare the fine structure of vitrified cumulus cells of human mature oocyte without oocyte and cumulus cells of human mature oocytes with oocyte were vitrified and ultrastructural changes using transmission electron microscopy (TEM). Results: The ultrastructural changes cumulus cell showed: Increasing amount of lipid droplets and cytoplasm has become non-homogeneous, the number of vesicles and smooth endoplasmic reticulation membranes have been increased in vitrified cumulus cell, cytoplasm membrane have been intact in cumulus cell without oocyte but cytoplasm membrane and nucleus membrane have been damage in cumulus cell with oocyte and all of the organelles Scattered in the surrounding oocyte. Conclusion: Vitrification damaged the organelles in cumulus cells of intact human mature oocytes. Therefore, it is recommended to vitrify Mil oocytes free of cumulus cells.
ACCESSION #
96841559

 

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