TITLE

Adoptive transfer of in vitro and pregnancy-induced CD4+CD25+ regulatory T cells prevent fetal rejection in abortion-prone mice

AUTHOR(S)
Rezaeenia, S.; Idali, F.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p33
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: During pregnancy the mother tolerates an allograft expressing paternal antigens (the fetus), which requires substantial changes in immune regulation over a programmed period of time. CD4+CD25+ T cells play a major role in tolerating conceptus antigens and therefore may contribute to the maintenance of pregnancy. Here, we investigated whether fetal rejection could be prevented by adoptive transfer of Tregs from in vitro generated or isolated from normal pregnant mice. Materials and Methods: Isolation of Mouse Mononuclear Cells was done with ficoll separation protocols and CD4+ T cells was purified on the MACS Cell Separator. Purified naive T cells were stimulated in anti-CD3 coated plates and soluble anti-CD2s in the presence of TGF-b, retinoic acid and IL-2 for 4 days. Induction of regulatory T cells was surveyed by flow cytometric analysis. CBA/J female mated with DBA/2J male mice (abortion model) and with BALB/c male (control mice). In addition, CD4+CD25+ Tregs freshly isolated from CBA/J pregnant mice (CBA/J x BALB/c). Pregnant CBA/J mice (CBA/J x DBA/2J) were injected intravenously with the freshly isolated or in vitro generated Tregs (21103 cell per mouse) on day 1- 4 of pregnancy. Results: In vitro stimulation of naive T cells resulted in up to 70% Foxp3 expression. The purity of isolated CD4+CD25+ T cells were estimated around 98%. Adoptive transfer of invitro generated Tregs in day 1 -4 of gestation resulted in 89% pregnancy rate. Fetal rejection completely prevented by adoptive transfer of Tregs from normal pregnant mice. Conclusion: We suggest that generated Tregs could be seen as therapeutic method in prevention of abortion.
ACCESSION #
96841558

 

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