TITLE

hCG regulates human endometrial epithelial cell adhesion through L-selectin ligand, MECA- 79

AUTHOR(S)
Kabir-salmani, M.; Nejatbakhsh, R.; Hosseini, A.; Dimitriadis, E.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p32
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Molecular interactions at the human embryo-endometrium interface play critical roles during embryo implantation. L-selectin and its carbohydrate ligand are important in the human embryo-endometrial apposition stage and blastocyst-derived hCG plays a significant roles during implantation. We aimed to determine the effects of hCG on the L-selectin ligand (MECA-79) in implantation using an in vitro co-culture model. Materials and Methods: Human choriocarcinoma cell (JEG-3) spheroids co-cultured with the human endometrial epithelial cell line (HES) were used to model blastocyst-endometrial interactions. Results: Our findings demonstrated that hCG increased the attachment of trophoblast-like spheroids to HES cells in a concentration-dependent manner. HESC cells treated with hCG at 1 and 5 IU/ml did not affect spheroid adhesion, however when cells were treated with 50 IU/ml hCG there was a significant increase in adhesion compared to control. MECA-79 is produced both by human endometrial epithelial cells and HES cells and addition of a MECA-79 blocking antibody during spheroid-HES co-cultures decreased spheroid adhesion to HES cells compared to control. Addition of the MECA-79 neutralising antibody to hCG treated HES cells significantly reduced spheroid attachment to HES cells compared to the hCG alone treatment group. These data demonstrate that hCG increased JEG-3 spheroid to HES cells and further that this occurred via MECA-79. Conclusion:These data is the first to demonstrate that hCG and the L-selectin ligand, MECA-79 regulated endometrial epithelial cell adhesion and extends our knowledge in embryo-maternal interactions during implantation. It suggests that targeting endometrial MECA-79 may be useful to facilitate implantation.
ACCESSION #
96841556

 

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