Goat spermatogonial xenotransplantation into the mouse testes

Heidari, B.; Naderi, M. M.; Farab, M.; Bebzadi, B.; Borjian-Boroujeni, S.; Sarvari, A.; Akhondi, M. M.; Shirazi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p32
Academic Journal
Introduction: Germ cell transplantation apart from its application as an alternative strategy in producing transgenic livestock with higher efficiency has a potential application in individuals with azoospermia and in pre-pubertal males. The present study was aimed to investigate transplantation efficiency of goat germ cells into the mouse testes as a recipient of xenogenic sperm. Materials and Methods: One month old goat testes were subjected to two-step digestion method and the spermatogonia were purified by using discontinuous percoll density gradients. Adult C57BL/6 mice, 6 weeks after receiving an injection of busulfan (30 mg/kg) to deplete endogenous germ cells, were used as recipient mice. The spermtogonia (450×103 cells/30μl/each injection) was transplanted into the rete testis of one testis of each recipient mice; the other testis served as control. Testis capsular thickness, tubular diameter and cell viability were evaluated before and after injection. The testes of busulfan-injected mice were recovered, fixed, and examined for histological and immunohistochemical staining (using an antibody against PGP9.5) after 80 days. Results: The capsular thickness was increased and the walls of the majority of the seminiferous tubules became thinner after busulfan treatment because of the depletion of premeiotic and meiotic germ cells. Donor goat spermatogonia, PGP.5 positive round cells with spherical big nucleus, were able to survive and colonize in depleted recipient's testis after 80 days. Conclusion: Mice can serve as a suitable model for development and evaluation of spermatogonial transplantation techniques in goat.


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