TITLE

Goat spermatogonial xenotransplantation into the mouse testes

AUTHOR(S)
Heidari, B.; Naderi, M. M.; Farab, M.; Bebzadi, B.; Borjian-Boroujeni, S.; Sarvari, A.; Akhondi, M. M.; Shirazi, A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p32
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Germ cell transplantation apart from its application as an alternative strategy in producing transgenic livestock with higher efficiency has a potential application in individuals with azoospermia and in pre-pubertal males. The present study was aimed to investigate transplantation efficiency of goat germ cells into the mouse testes as a recipient of xenogenic sperm. Materials and Methods: One month old goat testes were subjected to two-step digestion method and the spermatogonia were purified by using discontinuous percoll density gradients. Adult C57BL/6 mice, 6 weeks after receiving an injection of busulfan (30 mg/kg) to deplete endogenous germ cells, were used as recipient mice. The spermtogonia (450×103 cells/30μl/each injection) was transplanted into the rete testis of one testis of each recipient mice; the other testis served as control. Testis capsular thickness, tubular diameter and cell viability were evaluated before and after injection. The testes of busulfan-injected mice were recovered, fixed, and examined for histological and immunohistochemical staining (using an antibody against PGP9.5) after 80 days. Results: The capsular thickness was increased and the walls of the majority of the seminiferous tubules became thinner after busulfan treatment because of the depletion of premeiotic and meiotic germ cells. Donor goat spermatogonia, PGP.5 positive round cells with spherical big nucleus, were able to survive and colonize in depleted recipient's testis after 80 days. Conclusion: Mice can serve as a suitable model for development and evaluation of spermatogonial transplantation techniques in goat.
ACCESSION #
96841555

 

Related Articles

  • TAF4b is Required for Mouse Spermatogonial Stem Cell Development. Lovasco, Lindsay A.; Gustafson, Eric A.; Seymour, Kimberly A.; Rooij, Dirk G.; Freiman, Richard N. // Stem Cells (Wiley-Blackwell);Apr2015, Vol. 33 Issue 4, p1267 

    Long-term mammalian spermatogenesis requires proper development of spermatogonial stem cells (SSCs) that replenish the testis with germ cell progenitors during adult life. TAF4b is a gonadal-enriched component of the general transcription factor complex, TFIID, which is required for the...

  • You Are When You Eat. ABERLIN, MARY BETH // Scientist;Sep2013, Vol. 27 Issue 9, p13 

    An introduction to the journal is presented in which the editor discusses different reports in the issue including an article on night shift workers, lack of organs availability for organ transplantation and xenotransplantation.

  • Not a solution. Taylor, K. // BMJ: British Medical Journal (Overseas & Retired Doctors Edition;2/13/2010, Vol. 340 Issue 7742, p329 

    A letter to the editor is presented about xenotransplantation as a means to deal with organ failure in patients.

  • WHEN SHOULD PRECAUTION PREVAIL? INTERESTS IN (PUBLIC) HEALTH, THE RISK OF HARM AND XENOTRANSPLANTATION. Fovargue, Sara; Ost, Suzanne // Medical Law Review;Autumn2010, Vol. 18 Issue 3, p302 

    The article explores the proper reconciliation of private benefit and public risk with regards to the context of xenotransplantation. It discusses xenotransplantaion and its microbiological barriers, the pre-clinical and clinical trials and the regulations in the United Kingdom (UK) on...

  • Xeno-Transplantation: The Ultimate Organ Factory.  // Trends Magazine;Aug2011, Issue 100, p20 

    The article presents the anticipated ramifications of the emergence of xenotransplantation which is described as a surgical procedure of transferring tissues and organs from one species to another species. It predicts that the commercialization of blastocyst complementation will have an impact...

  • Adoptive Transfer With In Vitro Expanded Human Regulatory T Cells Protects Against Porcine Islet Xenograft Rejection via Interleukin-10 in Humanized Mice. Shounan Yi; Ming Ji; Jingjing Wu; Xiaoqian Ma; Phillips, Peta; Hawthorne, Wayne J.; O'Connell, Philip J. // Diabetes;May2012, Vol. 61 Issue 5, p1180 

    T cell-mediated rejection remains a barrier to the clinical application of islet xenotransplantation. Regulatory T cells (Treg) regulate immune responses by suppressing effector T cells. This study aimed to determine the ability of human Treg to prevent islet xenograft rejection and the...

  • The Establishment of Patient-derived Lung Cancer Xenograft Models by Cancer Tissue Transplant Directly. TANG Shi-cong; PAN Hong // Journal of Kunming Medical University / Kunming Yike Daxue Xueba;2014, Vol. 35 Issue 11, p169 

    Lung cancer is a threat to human health. It will be a significant tool for studying on lung cancer to establish a patient-derived lung cancer xenograft model. There are three approaches to establishing a lung cancer xenograft model, lung cancer cell culturing, lung cancer tissues transplant and...

  • xenotransplantation. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p809 

    A definition of "xenotransplantation" is presented. It refers to the organ transplantation from one species into another as in transplanting the organs of a pig into human beings. Genetic engineering research on the embryos of pigs are being conducted at present with the aim to produce animals...

  • FSH prevents depletion of the resting follicle pool by promoting follicular number and morphology in fresh and cryopreserved primate ovarian tissues following xenografting. von Sch”nfeldt, Viktoria; Chandolia, Ramesh; Ochsenkhn, Robert; Nieschlag, Eberhard; Kiesel, Ludwig; Sonntag, Barbara // Reproductive Biology & Endocrinology;2012, Vol. 10 Issue 1, p98 

    Background: Cryopreservation and transplantation of ovarian tissue is one option for re-establishing ovarian function, but optimal conditions for graft sustainment and follicular survival are still considered experimental. The present study aims to analyze the effect of FSH treatment on the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics