TITLE

Daidzein and Trifolium pratense hydroalcoholic extract negatively affected the testis structure and functions

AUTHOR(S)
Ghaffari-Sadaghiani, Z.; Malekinejad, H.; Razi, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p30
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Red clover (Trifoliumpratense) belongs to the plant family Leguminosae, which its estrogenic effects long before have been reported. Besides its beneficial effects, there are increasing evidence indicating phytoestrogens related detrimental effects in female reproductive system including breeding problems of sheep grazing on red clover pastures and fertility failure in cattle fed with red clover silage. During the last decade, exclusive debates and controversial studies were conducted to show the potential detrimental effects of phytoestrogens on male reproductive system. We in the present study aimed to show any potential effects of daidzein as a known phytoestrogen and hydro alcoholic extract of red clover flowers on the testis structure and the serum level of testosterone in adult male rats. Materials and Methods: The animals in five groups were exposed against saline (control group), daidzein (10 mg/kg, orally) and/or various dose levels of red clover extract (20, 40 and 80 mg/kg, b.w. orally) for 28 days. Results: Our results showed that 28 days exposure to 10 mg standard daidzein and/or high dose of red clover flowers extract resulted in 6- and 5-fold increase of germinal epithelium dissociation, respectively, when compared to the control group. At the same time germinal epithelium height and seminiferous tubules diameter were reduced in test groups significantly (p<0.05) in a dose-dependent fashion. Determination of serum level of testosterone confirmed the fact that the lower leydig cells number, the lower testosterone concentration. Conclusion: Our data suggest that phytoestrogens and red clover extract with known phytoestrogens do have negative effects on male reproductive system including structural and functional disorders.
ACCESSION #
96841551

 

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