Open versus closed oocyte vitrification system: effect on survival rate and spindle visualization

Agha-Rahimi, A.; Halvaei, I.; Anvari, M.; Ashourzadeh, S.; Khoradmehr, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p30
Academic Journal
Introduction: Vitrification has been successfully applied in the cryopreservation of oocytes and embryos. Prevention of cross contamination is critical in vitrification procedure that is possible with closed system. But few reports have been published regarding oocyte vitrification in closed systems. The aim of this study was evaluate the effect of a closed and open vitrification approach for oocyte cryopreservation on survival rate and spindle visualization Materials and Methods: 87 vitrification-warming cycles were performed on Mil mouse oocyte. Cryotop were used for open system and Rapid-iâ„¢ Vitrification System was used for closed system. Results: Survival rate was significant different in closed system and open system (60.9% versus 90.2%, p<0.001). But no statistically significant differences were observed in spindle visualization rates between the closed and open groups (60.0% vs. 46.3%, p=0.15). Conclusion: Closed system for vitrication did not adverse effect on spindle visualization in Mil oocyte, but this system had lower survival rate than open system.


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