TITLE

Comparision of stress and apoptosis-related genes expression between vitrified and fresh human GV oocytes after in-vitro maturation

AUTHOR(S)
Shahedi, A.; Yeganeh, F.; Hosseini, A.; Khalili, M. A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p29
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: The aim of this study was to assess the effects of vitrification on maturation as well as apoptotic, DNA methyl transferase and stress genes expression of immature human oocytes retrieved from ICSI cycles. Materials and Methods: 213 immature GV oocytes obtained from infertile patients and divided into two groups: (I) GV oocytes (n=106) matured in-vitro (fIVM), and (II) GV oocytes (n=107) that were first vitrified, then warmed and matured in-vitro (vIVM). Ham's F10 supplemented with FSH+LH plus human follicular fluid used as the maturation media. After 36 hrs of incubation the oocytes assessed for nuclear maturation and quantitative Real Time PCR used for expression patterns of stress (Sodl and Hsp70), DNA methyl transferase (Dnmtl) and apoptosis-related (Bc12 and Bax) genes. Results: The percentage of oocyte maturation was reduced (p<0.001) in vIVM (50.52%) as compared to flVM oocytes (85.84%). The percentages of degeneration were significantly higher in vIVM compare to flVM group (41.05% vs. 4.71%). Analysis of gene expression by real time PCR showed higher expression of stress and apoptosis-related genes in vIVM compared to the fIVM group. However DNMT1 expression indicated reduction in vIVM. Conclusion: IVM process was significantly impared in the virtified immature oocytes compare to fresh oocytes. Additionally, the expression of stress and apoptosis related genes increased after vitrification of immature human oocytes. The differential expression of apoptosis and stress related genes profile may be usefull to evaluate effectiveness of cryopreservation program for immature human oocytes.
ACCESSION #
96841548

 

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