Comparision of stress and apoptosis-related genes expression between vitrified and fresh human GV oocytes after in-vitro maturation

Shahedi, A.; Yeganeh, F.; Hosseini, A.; Khalili, M. A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p29
Academic Journal
Introduction: The aim of this study was to assess the effects of vitrification on maturation as well as apoptotic, DNA methyl transferase and stress genes expression of immature human oocytes retrieved from ICSI cycles. Materials and Methods: 213 immature GV oocytes obtained from infertile patients and divided into two groups: (I) GV oocytes (n=106) matured in-vitro (fIVM), and (II) GV oocytes (n=107) that were first vitrified, then warmed and matured in-vitro (vIVM). Ham's F10 supplemented with FSH+LH plus human follicular fluid used as the maturation media. After 36 hrs of incubation the oocytes assessed for nuclear maturation and quantitative Real Time PCR used for expression patterns of stress (Sodl and Hsp70), DNA methyl transferase (Dnmtl) and apoptosis-related (Bc12 and Bax) genes. Results: The percentage of oocyte maturation was reduced (p<0.001) in vIVM (50.52%) as compared to flVM oocytes (85.84%). The percentages of degeneration were significantly higher in vIVM compare to flVM group (41.05% vs. 4.71%). Analysis of gene expression by real time PCR showed higher expression of stress and apoptosis-related genes in vIVM compared to the fIVM group. However DNMT1 expression indicated reduction in vIVM. Conclusion: IVM process was significantly impared in the virtified immature oocytes compare to fresh oocytes. Additionally, the expression of stress and apoptosis related genes increased after vitrification of immature human oocytes. The differential expression of apoptosis and stress related genes profile may be usefull to evaluate effectiveness of cryopreservation program for immature human oocytes.


Related Articles

  • Notch signaling pathway in cumulus cells can be a novel marker to identify poor and normal responder IVF patients. Tanriverdi, Gamze; Denir, Secnur; Ayla, Sule; Bilir, Ayhan; Oktar, Huseyin; Cepni, Ismail; Irez, Tulay // Journal of Assisted Reproduction & Genetics;Oct2013, Vol. 30 Issue 10, p1319 

    Purpose: To identify expression of Notch signaling proteins and its ligands in human cumulus cells which were obtained by follicle aspiration and to compare the differences of this protein expression between the normal and poor responder patients. Methods: 47 patients who applied to the assisted...

  • Should fertile people have access to in vitro fertilisation? Dawson, Karen; Singer, Peter // BMJ: British Medical Journal (International Edition);1/20/90, Vol. 300 Issue 6718, p167 

    Examines the proposals for in vitro fertilization to infertile couples. Objections of the Roman Catholic church against in vitro fertilization; Efficacy of the procedure; Control of the chances for multiple birth.

  • Miracle-Baby Bills: Who Should Pay Up?  // Glamour;Mar99, Vol. 97 Issue 3, p224 

    Editorial. Comments on the insurance coverage for infertility treatments in the United States (US). Details on the June 1998 US court decision on infertility treatments; Comments of Pennsylvania state senator, Lisa Boscola on infertility treatments policy in the US; Costs of in vitro...

  • Effectiveness of treatment for infertility. Lilford, Richard J.; Dalton, Maureen E. // British Medical Journal (Clinical Research Edition);7/18/1987, Vol. 295 Issue 6591, p155 

    Examines the effectiveness of several treatment for infertility. Causes of infertility; Reasons for the poor implantation rates with in vitro fertilization; Benefits of hormonal treatment for the nebulous diagnosis of corpus luteal insufficiency.

  • Hope floats for childless couples in city of lakes.  // South Asian Post;8/16/2012, p17 

    The article focuses on a medical centre in Udaipur in Rajasthan, India which offers facilities for test tube fertilization for infertile couples.

  • Baby Steps: Having the Child I Always Wanted (Just Not as I Expected). Lindsey, Brandi // International Journal of Childbirth Education;Jan2014, Vol. 29 Issue 1, p92 

    The article reviews the book "Baby Steps: Having the Child I Always Wanted (Just Not as I Expected)," by Elisabeth Rohm.

  • The anguish of infertility. Beales, Elizabeth; Fallows, Carol // Australia's Parents;Jun/Jul96, Issue 93, p65 

    Recounts the experience of Leanne Browne and her family in dealing with infertility and their efforts to have a child. Struggle with infertility; In vitro fertilization (IVF) efforts; Success of her seventh IVF effort; Process behind IVF. INSET: Infertility treatments..

  • Induced abortions previous to IVF: an epidemiologic register-based study from Finland. E. Hemminki; R. Klemetti; T. Sevón; M. Gissler // Human Reproduction;Jun2008, Vol. 23 Issue 6, p1320 

    BACKGROUND The purpose of this study was to identify how many women treated for infertility had an abortion history, as well as when those abortions were carried out, and for what reasons. METHODS Data on all women treated in Finland from 1996–1998 for infertility either with IVF (n =...

  • Reply: CDC analysis of ICSI/autism: association is not causation. Kissin, Dmitry M; Zhang, Yujia; Boulet, Sheree L; Fountain, Christine; Bearman, Peter; Schieve, Laura; Yeargin-Allsopp, Marshalyn; Jamieson, Denise J // Human Reproduction;Jul2015, Vol. 30 Issue 7, p1746 

    No abstract available.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics