TITLE

Variable localization of TLRs in human fallopian tube epithelial cells

AUTHOR(S)
Amjadi, F.; Zandieh, Z.; Salehi, E.; Aflatoonian, A.; Fazeli, A.; Aflatoonian, R.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p28
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Infections of the female reproductive tract may contribute to infertility to a various extent depending on the site of inflammation, especially in fallopian tube. Histologically, fallopian tube is formed by a ciliated and non-ciliated epithelium. The immune system related to epithelial cells of the FRT provides the first reaction to sexually transmitted pathogens. Recognition of these pathogens is attributed to the family of Toll like receptors (TLR) as a major part of the innate immune system. This study clarifies the different expression and localization of the TLRs in fallopian tube epithelial cells. Materials and Methods: We firstly investigated TLRs localization in fallopian tube epithelial cells by immunostaining, surprisingly we found the intensity of staining was not equal in epithelial cells, then after primary cell culture of fallopian tube epithelial cells was performed. Ring cloning was used to isolate colonies of ciliated from non- ciliated epithelial cells and then the expression of TLR1-10 was examined by Quantitative real time PCR. Results: We found TLR1-10 to be expressed in Fallopian tube epithelial cells, our studies revealed enriched localization of TLRs in Fallopian tube ciliated epithelial cells. We showed that TLRs expression in fallopian cells, with a higher level in the cilia cells versus non-ciliated cells (p<0.05). Conclusion: TLRs is localized in human Fallopian tube epithelial cells, particularly in the ciliated cells, and is likely to have a cilia-specific role in protection of reproductive tract.
ACCESSION #
96841546

 

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