TLRs are a new insight in physiology of maternal interaction with sperm

Zandieh, Z.; Ashrafi, M.; Amanpour, S.; Aflatoonian, R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p28
Academic Journal
Introduction: Toll Like Receptors are the major compartment of innate immune system. It was revealed that the TLRs have physiological relevance in ovulation, sperm capacitation, fertilization and pregnancy. So, in this study the expression of TLRs in human fallopian tube cell line under effect of the sperm was evaluated.The effect of sperm physical contact with fallopian tube epithelial layer or just presence of sperm without physical contact on TLR expression also was evaluated. Materials and Methods: Sperms from 8 healthy donors were added in 4 groups for 24 hours: 1- sperm group: coculture of normal sperm with OE-E6/E7 cell line, 2-sperm + membrane group: coculture of normal sperm with OE-E6/E7 by using membrane supports, to prevent contact between the epithelial cells and spermatozoa, 3-Control group and 4- control + membrane group. Afterward, Toll like receptors expressions were compared in these groups by Q- PCR. In the next part, the level of TLR, their adaptor molecule and cytokines were compared with Q- PCR in sperm and control group and supernatant were used for ELISA assay. Results: OE-E6/E7 expressed TLR 1-6 genes and proteins. The mean relative expression of TLR 2, 4, 5 and 6 in sperm+ membrane group were higher significantly in compare with 3 other groups. TLRs expressions in OE-E6/E7 under effect of sperm were significantly increased in TLR3 and TLR5. Also, their signaling pathways as well as cytokine production were higher in sperm group than control (p<0.05). Conclusion: TLRs are a new insight in physiology of maternal interaction with sperm.


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