TITLE

Does women's age influence zona pellucida birefringence of metaphase II oocytes in in-vitro maturation program?

AUTHOR(S)
Omidi, M.; Khalili, M.; Nahangi, H.; Ashourzadeh, S.; Rahimipour, M.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p28
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of PolScope, it has become possible to evaluate zona pellucida (ZP) characteristics as a parameter of oocyte quality. Moreover, quality of oocytes can be influenced by many factors, such as patient's age. The PolScope system is a non-invasive technique to assess birefringent structures such as the meiotic spindle and ZP in living oocytes. The aim was to determine the influence of the woman's age on ZP birefringence, a sign of oocyte quality, and morphology of in-vitro matured human oocytes using non-invasive polarized light (PolScope) microscopy. Materials and Methods: ZP birefringence and morphology were determined in 105 retrieved oocytes from 58 women undergoing ICS1 in two age groups (⩾30 years and <30 years). The immature oocytes were selected and after IVM, the quality of metaphase II (Mil) oocytes was assessed. The oocytes abnormalities were classified as intracytoplasmic and extracytoplasmic abnormalities. Results: Oocyte maturation rates were significantly reduced in >30 year's women (56%) in comparison with other age group (80.7%). In addition, the ZP birefringence was significantly higher in Mil oocytes in the younger group compared with the older group (76.2% vs. 38.1%; p=0.00). Following morphologic assessment, the rates of oocytes with extracytoplasmic (p=0.02) and both abnormalities (extra- and intracytoplasmic) (p=0.01) were higher in aged versus the younger women. Conclusion: There was a positive relationship between advanced maternal age with decreased ZP birefringence and oocyte morphological quality in in-vitro matured human oocytes.
ACCESSION #
96841544

 

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