TITLE

Vitamin C can protect spermatozoa from detrimental effects of diabetes mellitus; effects on sperm parameters and DNA integrity using different cytochemical tests

AUTHOR(S)
Mangoli, E.; Talebi, A.; Halvaei, I.; Taheri, F.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p26
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: The main goal was to evaluate the protective effects of vitamin C on sperm parameters, chromatin condensation and apoptosis in experimentally-induced diabetic mice. Also the correlation between different DNA integrity tests was assessed. Materials and Methods: 21 adult male Syrian mice were divided randomly into 3 groups (n=7). Group 1 were included diabetic mice that received a single dose of Streptozocin (STZ) (200 mg/kg) intra-peritoneal (IP), group 2 were diabetic mice that received vitamin C (10 mg/kg/daily, IP) and group 3 were considered as controls. After 35 days, the sperm samples were collected from cauda epididymis and analysed for sperm count, progressive motility, and morphology. Aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), acridine orange (AO), and terminal transferas-emediateddeoxyuridine triphosphate-biotin end labelling (TUNEL) tests were used in order to evaluate sperm DNA status. Results: The data showed that diabetes can significantly reduce sperm count, motility as well as normal morphology and vitamin C can attenuate these effects (P<0.0001). Regarding sperm DNA quality, the data showed that there is a significant increase in AO TB and TUNEL spermatozoa in group 1 compared to others. The rate of spermatozoa with abnormal DNA were significant reduced in group 2 (P<0.0001). The significant positive correlation was seen between TUNEL and TB, AO (Pearson correlation: 0.86 and 0.82, respectively, P<0.0001) and between AO and TB as well (Pearson correlation: 0.87, P<0.0001). Conclusion: Vitamin C can be used as a protective agent in diabetic patients to improve sperm parameters especially sperm DNA integrity.
ACCESSION #
96841540

 

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