Vitamin C can protect spermatozoa from detrimental effects of diabetes mellitus; effects on sperm parameters and DNA integrity using different cytochemical tests

Mangoli, E.; Talebi, A.; Halvaei, I.; Taheri, F.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p26
Academic Journal
Introduction: The main goal was to evaluate the protective effects of vitamin C on sperm parameters, chromatin condensation and apoptosis in experimentally-induced diabetic mice. Also the correlation between different DNA integrity tests was assessed. Materials and Methods: 21 adult male Syrian mice were divided randomly into 3 groups (n=7). Group 1 were included diabetic mice that received a single dose of Streptozocin (STZ) (200 mg/kg) intra-peritoneal (IP), group 2 were diabetic mice that received vitamin C (10 mg/kg/daily, IP) and group 3 were considered as controls. After 35 days, the sperm samples were collected from cauda epididymis and analysed for sperm count, progressive motility, and morphology. Aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), acridine orange (AO), and terminal transferas-emediateddeoxyuridine triphosphate-biotin end labelling (TUNEL) tests were used in order to evaluate sperm DNA status. Results: The data showed that diabetes can significantly reduce sperm count, motility as well as normal morphology and vitamin C can attenuate these effects (P<0.0001). Regarding sperm DNA quality, the data showed that there is a significant increase in AO TB and TUNEL spermatozoa in group 1 compared to others. The rate of spermatozoa with abnormal DNA were significant reduced in group 2 (P<0.0001). The significant positive correlation was seen between TUNEL and TB, AO (Pearson correlation: 0.86 and 0.82, respectively, P<0.0001) and between AO and TB as well (Pearson correlation: 0.87, P<0.0001). Conclusion: Vitamin C can be used as a protective agent in diabetic patients to improve sperm parameters especially sperm DNA integrity.


Related Articles

  • Influence of 3-hour abstinence on sperm DNA fragmentation and the maturity of sperm chromatin. Lakpour, N.; Amirjannati, N.; Shani, S.; Akhondi, M.; Fathi, Z.; Bolourzadeh, M.; Jahromi, N. Saadaei; Sadeghi, M. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p119 

    Introduction: Repeated ejaculation was suggested that accompanies with decrease in sperm DNA fragmentation (SDF). Therefore, we conducted this study to evaluate efficacy of 3-hour abstinence in reducing SDF and sperm chromation maturity in fertile men. Materials and Methods: Thirty seven fertile...

  • Method to predict the chance of developing a male profile out of mixtures of male and female DNA. Laberke, Patrick; Grossenbacher, Rahel; Hausmann, Roland; Balitzki, Beate // International Journal of Legal Medicine;Jan2012, Vol. 126 Issue 1, p157 

    In forensic examination it is a standard to take vaginal swabs from victims of sexual assault for further molecular genetic analysis. Laboratories then are usually confronted with mixtures of lots of female and only a small amount of male DNA. Nowadays it is possible to work with specific Y...

  • Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice. Rahimipour, Marzieh; Talebi, Ali Reza; Anvari, Morteza; Sarcheshmeh, Abolghasem Abbasi; Omidi, Marjan // Iranian Journal of Reproductive Medicine;May2014, Vol. 12 Issue 5, p307 

    Background: Saccharin is an artificial non-caloric sweetener that used to sweeten products such as drinks, candies, medicines, and toothpaste, but our bodies cannot metabolize it. Sodium saccharin is considered as an important factor in tumor promotion in male rats but not in humans. Objective:...

  • Ejaculate Oxidative Stress Is Related with Sperm DNA Fragmentation and Round Cells. Iommiello, Valeria Maria; Albani, Elena; Di Rosa, Alessandra; Marras, Alessandra; Menduni, Francesca; Morreale, Giovanna; Levi, Shanti Lia; Pisano, Benedetta; Levi-Setti, Paolo Emanuele // International Journal of Endocrinology;2/23/2015, Vol. 2015, p1 

    Oxidative stress (OS) plays an essential role in male infertility aetiology by affecting sperm quality, function, and also the integrity of sperm DNA. The assessment of oxidative stress in semen may be an important tool to improve the evaluation of sperm reproductive capacity. The purpose of...

  • DNA fragmentation in human sperm after magnetic-activated cell sorting. Bucar, Sara; Gonçalves, Ana; Rocha, Eduardo; Barros, Alberto; Sousa, Mário; Sá, Rosália // Journal of Assisted Reproduction & Genetics;Jan2015, Vol. 32 Issue 1, p147 

    Purpose: As fertilization with unselected apoptotic spermatozoa may contribute to failures in assisted reproductive techniques, it has become essential to remove this type of sperm in order to increase the success rates. Magnetic-activated cell sorting (MACS) is a sperm preparation technique...

  • ENU-Induced Mutagenesis in Grass Carp (Ctenopharyngodon idellus) by Treating Mature Sperm. Xia-Yun Jiang; Cheng-Fei Sun; Quan-Gen Zhang; Shu-Ming Zou // PLoS ONE;2011, Vol. 6 Issue 10, p1 

    N-ethyl-N-nitrosourea (ENU) mutagenesis is a useful approach for genetic improvement of plants, as well as for inducing functional mutants in animal models including mice and zebrafish. In the present study, mature sperm of grass carp (Ctenopharyngodon idellus) were treated with a range of ENU...

  • Dichotomous Effect of Caffeine, Curcumin, and Naringenin on Genomic DNA of Normal and Diabetic Subjects. Chattopadhyay, Debarati; Somaiah, Ashok; Raghunathan, Divya; Thirumurugan, Kavitha // Scientifica;2014, p1 

    Nutraceutical compounds show antioxidant and prooxidant properties under stress conditions like cancer, diabetes, and other diseases. The objective of this study is to find the dichotomic behavior of caffeine, curcumin, and naringenin on DNA of diabetic and normal subjects in the presence and...

  • Sitagliptin Successfully Ameliorates Glycemic Control in Werner Syndrome With Diabetes. KOBAYASHI, KAZUKI; KAWAMURA, HARUKIYO; YOKOTE, KOUTARO; KITAMOTO, TAKUMI; TAKEMOTO, MINORU; FUJIMOTO, MASAKI; ISHIKAWA, TAKAHIRO; ONISHI, SHUNICHIRO; OKABE, EMIKO; ISHIBASHI, RYOICHI // Diabetes Care;Dec2012, Vol. 35 Issue 12, pe83 

    The article discusses case study of a 58-year-old Japanese woman with Werner Syndrome (WS). It has been informed that she exhibited graying and loss of hair, a hoarse voice, refractory skin ulcers, bilateral juvenile cataracts, dyslipidemia and diabetes and WS was confirmed by genomic DNA...

  • Lipsome-mediated uptake of exogenous DNA by Sahiwal cattle spermatozoa. Babu, Thomas V.; Pratheesh, M. D.; Anandlaxmi, N.; Amarnath, K. // Veterinary World;Oct2012, Vol. 5 Issue 10, p621 

    Aim: To investigate the influence of lipofection treatment and exogenous DNA uptake on the quality of sahiwal cattle spermatozoa. Materials and Methods: Semen collected from sahiwal bulls (n=7) were evaluated separately for color, volume, mass activity, concentration, motility and viability...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics