TITLE

Human spermatogonial stem cells tracking ex vivo

AUTHOR(S)
Zahiri, M.; Movahedin, M.; Mowla, S.; Noruzinia, M.; Nowroozi, M.; Amirjanati, N.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p25
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Evaluation of viability, proliferation and differentiation of human spermatogonial stem cells (hSSCs) and finally assessment of cell behavior after transplantation are main examples that help to predict their status in vivo. Materials and Methods: A control versus treatment study provide by 40 randomly selected mice. After the in vitro enrichment and proliferation of isolated hSSCs obtained from Testicular biopsies, they were transplanted to azoospermia mice models and after 4 months the weight of testes, spermatogenic cell numbers as well as epididymal sperm parameters were assessed and donor sperm were detected by human CatSper gene expression with quantitative PCR. Results: After one month of transplantation, Donor SSCs could home on the basal part of the seminiferous tubules. Weight testes, epididymal sperm number and also spermatogenic cell numbers in transplanted testes was significantly higher than other groups (p≤0.05). Expression profile of human CatSper after 16 weeks showed that the donor cells could involved in mice endogenous spermatogenesis. Conclusion: Our result was indicated that evaluation of transplanted testes is useful to predict hSSCs status in vivo.
ACCESSION #
96841538

 

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