Evaluation of sperm chromatin structure and morphology in different patterns of HOST positive sperm

Bassiri, F.; Tavalaee, M.; Nasr-Esfahani, M. H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p25
Academic Journal
Introduction: One of the effective methods for the treatment of infertility caused by the male factors is the ICSI. In this method, the embryologists select the best sperm based on its morphology and motility, and then, is injected into mature oocyte. However, this selection doesn't necessarily guaranty the sperm DNA integrity. Based on the existing relation between the membrane and the intracellular damage, the HOS test with the capability of assessing the membrane integrity, has been chosen as one of the methods for selecting normal sperm. In this test, the sperm are exposure hypo-osmotic condition. According to the difference between the membrane reactions, the swelling of the sperm tails occur in different pattern. Therefore the chromatin, apoptosis, morphology evaluations in different HOS patterns for selecting the best sperm has become the main purpose of this study. Materials and Methods: Semen samples were washed, exposed to hypotonic condition, then fixed and simultaneously assessed for abnormal morphology, DNA fragmentation and protamine deficiency using papanicoulau, TUNEL and CMA3 staining, in different HOST grades, respectively. For the assessment of membrane integrity or early apoptosis, the remaining semen samples were washed with Ca buffer and stained by Annexin V. Subsequently, Annexin stained samples were exposed to hypo osmotic conditions. Results: Obtained results of this study indicated the highest level of damage in the (a) and (g) patterns, and lowest damage level were in (d) and (c) patterns respectively.


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