TITLE

Comparison of septin14 expression in testes of patients with positive and negative testicular sperm retrieval

AUTHOR(S)
Barzi, N. Vahabi; Sabbaghian, M.; Onsory, Kh.; Hosseinifar, H.; Modarresi, T.; Nickhah, Z.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p24
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: The septins are a family of GTPase expressed in a variety species, ranging from unicellular yeasts to humans. In mammals, 14 septin genes have been identified so far. They have diverse cellular roles including polarity determination, vesicle trafficking, mitosis, and exocytosis. Disruption of septin functions has been implicated in the pathology of many diseases, including cancers, neurodegenerative diseases, and male infertility. Here, we study about one of the new member of septin family called septin14 that specially expressed in testis. SEPT 14 maps to 7pl 1.2 in humans. The objective of the study was to evaluate the expression pattern of Septin 14 in testis tissue of men with and without spermatogenic failure. Materials and Methods: Testicular tissues were obtained from biopsies of azoospermic men and subdivided into two groups: 10 patients with positive result in Testicular Sperm Extraction (TESE+) and 10 patients with negative result (TESE-). Total RNA was extracted using trizol reagent. Septin expression level was assessed by real-time reverse transcription-polymerase chain (RT-PCR) and it was normalized to expression of the GAPDH. Results: The testicular tissues of men with positive result of TESE had higher levels of septin 14 transcripts than those with negative results. According to pathology report tissues in negative group were subdivided to maturation arrest & Sertoli cell only groups. Conclusion: It was indicated that the expression levels of septin 14 are critical for normal spermatogenesis and decreased expression of this gene is associated with the pathogenesis of male infertility.
ACCESSION #
96841536

 

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