Comparison of septin14 expression in testes of patients with positive and negative testicular sperm retrieval

Barzi, N. Vahabi; Sabbaghian, M.; Onsory, Kh.; Hosseinifar, H.; Modarresi, T.; Nickhah, Z.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p24
Academic Journal
Introduction: The septins are a family of GTPase expressed in a variety species, ranging from unicellular yeasts to humans. In mammals, 14 septin genes have been identified so far. They have diverse cellular roles including polarity determination, vesicle trafficking, mitosis, and exocytosis. Disruption of septin functions has been implicated in the pathology of many diseases, including cancers, neurodegenerative diseases, and male infertility. Here, we study about one of the new member of septin family called septin14 that specially expressed in testis. SEPT 14 maps to 7pl 1.2 in humans. The objective of the study was to evaluate the expression pattern of Septin 14 in testis tissue of men with and without spermatogenic failure. Materials and Methods: Testicular tissues were obtained from biopsies of azoospermic men and subdivided into two groups: 10 patients with positive result in Testicular Sperm Extraction (TESE+) and 10 patients with negative result (TESE-). Total RNA was extracted using trizol reagent. Septin expression level was assessed by real-time reverse transcription-polymerase chain (RT-PCR) and it was normalized to expression of the GAPDH. Results: The testicular tissues of men with positive result of TESE had higher levels of septin 14 transcripts than those with negative results. According to pathology report tissues in negative group were subdivided to maturation arrest & Sertoli cell only groups. Conclusion: It was indicated that the expression levels of septin 14 are critical for normal spermatogenesis and decreased expression of this gene is associated with the pathogenesis of male infertility.


Related Articles

  • Single nucleotide polymorphisms in the SEPTIN12 gene may be associated with azoospermia by meiotic arrest in Japanese men. Miyamoto, Toshinobu; Tsujimura, Akira; Miyagawa, Yasushi; Koh, Eitetsu; Namiki, Mikio; Horikawa, Michiharu; Saijo, Yasuaki; Sengoku, Kazuo // Journal of Assisted Reproduction & Genetics;Jan2012, Vol. 29 Issue 1, p47 

    Purpose: To investigate the association between SEPTIN12 gene variants and the risk of azoospermia caused by meiotic arrest. Methods: Mutational analysis of the SEPTIN12 gene was performed using DNA from 30 Japanese patients with azoospermia by meiotic arrest and 140 fertile male controls....

  • Unilateral cryptorchidism appears to compromise paternity. Kuznar, Wayne // Urology Times;Feb98, Vol. 26 Issue 2, p22 

    Reports on the finding that compromised spermatogenesis is one possible explanation for decreased paternity among formerly unilateral cryptorchid men. Infertility rate of 10 percent among men who had surgical correction of an undescended testis a few months after birth to age 14; Higher...

  • Human genetics and disease: Mouse models of male infertility. Cooke, Howard J.; Saunders, Philippa T. K. // Nature Reviews Genetics;Oct2002, Vol. 3 Issue 10, p790 

    Spermatogenesis is a complex process that involves stem-cell renewal, genome reorganization and genome repackaging, and that culminates in the production of motile gametes. Problems at all stages of spermatogenesis contribute to human infertility, but few of them can be modelled in vitro or in...

  • Spermatogonial stem cell research results encouraging. Guttman, Cheryl // Urology Times;May2000, Vol. 28 Issue 5, p24 

    Reports that ongoing studies are providing researchers with an improved understanding of spermatogenesis that may lead to new approaches for male infertility management and gene therapy, according to David J. Cozzolino, a fellow in reproductive medicine and surgery. Research showing how...

  • Ooplasmic round spermatid nuclear injection procedures as an experimental treatment... Yamanaka, Keiko; Sofikitis, Nikolaos V. // Journal of Assisted Reproduction & Genetics;Jan97, Vol. 14 Issue 1, p55 

    Applies ooplasmic round spermatid nuclear injections for the treatment of nonobstuctive azoospermia. Includes the outcome of human ooplasmic round spermatid nuclear injections; Identification of cells isolated as round spermatids of stages 2 to 7 of spermiogenesis using electron microscopy and...

  • Examination of Idiopathic Infertility in the Presence of Normal FSH Levels.  // Fertility Weekly;1/14/2002, p16 

    Evaluates the hypophyso-gonadal axis by measuring the intratesticular concentrations of several steroids in relation with the serum hormonal status by researchers in France. Analysis on the role of testosterone and follicle stimulating hormone; Measurement of the steroid levels in testicular...

  • Polymorphisms of MSY2 Gene may be Linked to Impaired Spermatogenesis.  // Fertility Weekly;6/2/2008, p8 

    The article presents a study which assesses the potential relationship of MSY2 gene to idiopathic male infertility in China. The study reveals that allele C and G appeared to have a protective function against spermatogenic impairment. It concludes that MSY2 polymorphisms might be associated...

  • Patterns Of Testicular Histopathology In Men With Primary Infertility. Rashed, Mona M.; Ragab, Noha M.; Shalaby, Ahmad R.; Ragab, Waleed K. // Internet Journal of Urology;2008, Vol. 5 Issue 2, p11 

    Male infertility contributes to more than half of all cases of childlessness; yet, it is a reproductive health problem that is poorly studied and understood. The present study aims at the histopthological re-evaluation of the archived testicular biopsy specimen in order to delineate the...

  • Can sperm count increase in six to eight weeks? Sah, Purushottam // Internet Journal of Endocrinology;2010, Vol. 6 Issue 1, p1 

    Medical therapy for oligospermia is usually given for a minimum period of three months, so that it covers a complete spermatogenic cycle. One experimental study shows that spermatogenic cycles may be shorter, about sixty days, in men. Here clinical data of five oligospermic men are presented to...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics