TITLE

Increased DNA fragmentation in patients with partial globozoospermia

AUTHOR(S)
Rasouli, F.; Sabbaghian, M.; Hosseinifar, H.; Modarresi, T.; Nickhah, Z.; Gilani, M. Sadighi
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p24
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Infertility is estimated to affect up to 15% of couples of reproductive age. Globozoospermia is a rarely described morphologic disorder of spermatozoa observed in <1% of infertile men. Globozoospermia is characterized by the absence of acrosome in round-headed spermatozoa, leading to a complete inability to fertilize the oocyte, thus the affected males suffer from infertility. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated with teratozoospermia. The aim of this study was to evaluate the sperm DNA damage and protamine deficiency in men with partial globozoospermia and men with normal spermogram referred to Royan institute. Materials and Methods: Sperm samples were obtained from 24 patients with partial globozoospermia and 41 normozospermic men and semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA damage was assessed using Chromomycin A3 (CMA3) and terminal desoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling (TUNEL) assay, respectively. Results: Data analysis revealed that percentage CMA3 positivity (p<0.001) and TUNEL-positive spermatozoa (p=0.003) were higher in the individuals with partial globozoospermia than the men with normal spermogram. Conclusion: Several studies have attempted to correlate DNA fragmentation rate with sperm characteristics. However, there is just some case reports about DNA fragmentation rate in globozoosprmic men. This is cohort report of round-headed sperm that has shown an elevated number of sperm with protamine deficiency and DNA strand breaks compared to control group.
ACCESSION #
96841535

 

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