(+) Pulegone-induced damages in mice testicular tissue; evidence for RNA damage and apoptosis

Moradimoadab, Z.; Razi, M.; Shahrouz, R.; Malekinejad, H.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p23
Academic Journal
Introduction: Pulegone (PGN) is a natural organic compound in variety of plants that is widely used in different areas for flavoring foods, drinks, and dental products. However, it is known to impact stroidogenesis processes by affecting aromatase enzymes mRNA expression and synthesis. Present study aimed in order to evaluate PGN's impact on testicular tissue and sperm parameters in mice model. Materials and Methods: Twenty four mature mice were randomly divided into four groups as control-sham (received 0.3mL normal saline, ip) and test groups including: low dose (25 mg/kg, ip), medium dose (50 mg/kg, ip) and high dose (100mg/kg, ip)-PGN-received groups. After 35 days, serum levels of testosterone were evaluated. Germinal cells RNA damage, tubular differentiation (TDI), repopulation (RI) and spermiogenesis (SPI) were analyzed by epifluorescent and light microscopes, respectively. Moreover, sperm count, motility, chromatin condensation and DNA damage were assessed. Results: PGN decreased (p<0.05) serum level of testosterone and significantly (p<0.05) reduced the percentage of tubules with positive TDI, RI and SPI indexes. Epifluorescent analyses showed that PGN resulted in severe RNA damage in germinal epithelium. Sperm count, motility, chromatin decondensation and DNA disintegrity remarkably (p<0.05) elevated in PGN-treated animals in a dose dependent manner. Conclusion: Our data showed that, PGN exerts it impact partly via down-regulating the endocrine status of the testicles, which in turn considerably impacts the spermatogenesis and spermiogenesis processes. Ultimately, last impairment affects the sperm parameters. Defected chromatin condensation provokes the sperm DNA damage.


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