Evaluation of CD52 positive sperm in semen samples and effects on outcome of IUI procedure

Seifali, R.; Aboutorabi, R.; Shahverdi, A.; Ebrahimi, B.; Esmaeili, V.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p23
Academic Journal
Introduction: Proteins which should be located in sperm membrane have high impression in sperm maturation and in male fertility. CD52 is one of the antigens on sperm membrane and is a GPI (glycosylphosphatidylinositol) anchored protein that express on lymphocytes and epididymal cells. The aim of this study was to obtain any association between the percentage of CD52 positive sperm with semen parameters and also evaluation of correlation between the percentages of CD52 positive sperm with outcome of IUI. Materials and Methods: A total number of 120 semen samples were collected randomly from infertile couples who were undergoing IUI procedure. Samples were evaluated according to WHO 2010 guidelines. Sperm were washed and immunostaining with the monoclonal antibody CAMPATH-1G for CD52 then analyzed by flowcytometry. Results: This study indicates that mean percentage of CD52 expression was 79.25% in ejaculated sperm. The results show that there is a significant correlation between the percentage of CD52 expression and viability of spermatozoa (r=0.454, p=0.000). Furthermore, the percentage of CD52 expression and sperm motility were analyzed and a significant correlation between them was perceived. (r=0.597, p=0.000). Regarding to the results the area under the ROC curve is equal to 0.546 and CD52 is not a good predictor variable for fertility status (area=0.546, p=0.547). Conclusion: The results indicate that the correlation between the percentage of CD52 positive sperm with viability and motility was positively significant. However the results can not show any relation between the proportion of CD52 expression and pregnancy in couples candidate for IUI.


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