Is there any difference between commercial and home-made culture media on human sperm parameters after sperm preparation?

Nabi, A.; Khalili, M.; Halvaei, I.; Anbari, F.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p22
Academic Journal
Introduction: There are several techniques for sperm preparation in order to use in assisted reproductive technology. Several sperm preparation media such as commercial and home-made may be used in infertility clinics. To compare the effects of commercial and home-made human sperm preparation media on sperm parameters following direct swim-up method. Materials and Methods: ten normal semen samples were analyzed according to WHO guidelines. Makler chamber was used for evaluation of sperm count and motility. Sperm morphology was assessed using Giemsa staining method. Direct swim-up was supplied for sperm preparation. There were three groups in our study: a) sperm preparation medium from GYNEMED, b) Ham's f10 supplemented with 5 mg/ml human serum albumin and c) Ham's f10 supplemented with 10 mg/ml human serum albumin. Sperm parameters were evaluated at different time intervals: 0, 1, 3, 24 hr. Results: there were insignificant differences in sperm parameters after sperm preparation between different groups. Progressive motility after lh between commercial and home-media culture was 62.71±1.83% vs. 64±1.02% respectively. Also between commercial and home-media culture was no significant increase in progressive motility after 24h (23.95±0.7% vs. 22.45±1%, respectively). Conclusion: our data showed that there are no significant differences for conventional sperm parameters between commercial and home-made human sperm preparation media. It is suggested to compare sperm chromatin structure following different sperm preparation media.


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