Vitrification of human spermatozoa with seminal fluid and artificial seminal fluid

Agha-Rahimi, A.; Khalili, M.; Moradi, A.; Talebi, A.; Ghasemzadeh, J.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p22
Academic Journal
Introduction: Vitrification is a new method that has been recently introduced in ART. This study investigated the ability of patient's seminal fluid and artificial seminal fluid against cryo damage. These media were compared with routine medium (HTF supplemented with sucrose). Materials and Methods: Spermatozoa were isolated using the swim-up procedure performed using three different media: (a) HTF with 5% HSA and 0.25 M sucrose; (b) patient seminal fluid; (c) artificial seminal fluid. From each group, 30 ul suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by quick submerging the spheres in base medium (group a and b in HTF; group c in artificial seminal fluid) at 37°C with gentle agitation. Both sperm motility and viability were assessed pre-and post- vitrification. Motility was categorized into fast and slow (progressive), non- and immotile spermatozoa. Results: Viability of spermatozoa was not different significantly in three vitrified groups. The numbers of progressive motile spermatozoa were similar in three vitrified groups. But, fast progressive motile sperms were significantly higher in patients' seminal fluid and artificial seminal fluid (36.2±12.7 and 34.06±8.8, respectively), when compared with HTF supplemented with sucrose (18.4±9.2). Conclusion: Vitrification of human spermatozoa with patients' seminal fluid and artificial seminal fluid can effectively preserve the quality of motility in comparison with routine procedure.


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