Relationship of hyaluronic acid-bound spermatozoa with conventional semen parameters, lipid peroxidation, intracellular ROS, DNA fragmentation and mitochondrial membrane potential

Rashki Ghaleno, L.; Rezazadeh Valojerdi, M.; Chehrazi, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p21
Academic Journal
Introduction: This study was performed to evaluate association of hyaluronic acid - bound spermatozoa with conventional semen parameters, lipid peroxidation (LPO) , intracellular reactive oxygen species (ROS), DNA fragmentation (DF) and mitochondria membrane potential (MMP) . Materials and Methods: Semen samples were obtained from 99 patients who underwent seminal fluid evaluation at Royan Institute. The seminal plasma was separated for analysis of LPO and the sperm cells was employed for flowcytometric study of intracellular ROS, DF (sperm chromatin structure assay) and MMP. Results: The bound spermatozoa to hyaluronic acid showed the positive significant correlation with all conventional semen parameters consist of count (p<0.01), total motility (p<0.01), progressive motility (p=0.004), normal morphology (p<0.01), grade A, C of motility (respectively p<0.01, p=0.021) and the percentage of high DNA stain ability (HDS). In addition, it displayed a significant negative relationship with grade D of motility (p<0.01). However, after linear regression analysis, only normal morphology (p=0.001), grade A of motility (p=0.015) and HDS (0.021) effectively communicated with bound spermatozoa. Conclusion: The selection of spermatozoa by hyaluronic acid is strongly related to integrity of morphology, chromatin condensation and sperm maturity and there is no relationship with intracellular ROS and MMP level.


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