Investigation of ovine embryo development in the presence of FGF2

Moradi, M.; Riasi, A.; Ostadhosseini, S.; Hajian, M.; Hosseini, S.; Nasr-esfahani, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p19
Academic Journal
Introduction: Fibroblast growth factors are a great family of growth factors with variety of functions in vertebrate's including cell growth, survival and differentiation. FGFRs are tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs exist in vertebrates. FGF2is one of the FGFs detected in ovine uterus flushes, supposing its effects on embryo development. Materials and Methods: In vitro produced ovine embryos via IVF obtained at different stages (zygote, 2-4cell, 8-16 cell, morula, and blastocyst) for genetic analysis. In further investigations, from dayl, embryos were divided into two groups (control or 500 ng/ml FGF2) in serum-free conditions. FGF2 was present whole day's and different aspects of development were assessed until day 8. Results: All four types of FGFRs evinced dominant expression at morula stage while in the other stages FGFRs expression was in low levels. FGF2 supplementation did not significantly change the blastocyst formation rate and total cell numbers compared to control. Although FGFRs were present in ovine embryos, their expression were in low levels except in morula and it is unclear whether FGFRs are in adequate levels to transduce FGF2 signals in ovine embryos. Also it does not seem that FGF2 exerts any proliferative or developmentally amending effect on ovine embryos up to day 8.however, there is merit to investigate whether proliferative respond to FGF2 will appear from hatched blastocyst stage onward, one in which high proliferation rates prevail. Conclusion: We neither found aprominent effect of FGF2 on blastocyst formation rate nor proliferation in ovine embryos up to day 8 post fertilization.


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