Effects of angiotensin II addition to the culture media on Na+/K+/ATPase expression and subsequent developmental competence of ovine embryos

Naderi, M. M.; Heidari, B.; Sarvari, A.; Borjian Boroujeni, S.; Behzadi, B.; Akhondi, M. M.; Shirazi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p19
Academic Journal
Introduction: Presence of components of the renin-angiotensin system in mammalian ovaries and their involvement in ovarian physiology has been indicated. In the present study effects of angiotensin II (Ang II) addition to the maturation and embryo culture media on Na+/K+/ATPase expression and subsequent development of ovine embryos was evaluated. Materials and Methods: The abattoir-derived COCs (cumulus oocyte complexes) were randomly allocated into three experimental groups: I) IVM in medium supplemented with 1000 ng/mL Ang II; ii) IVM/IVF embryos followed by IVC wherein the resulting embryos were exposed to IVC medium supplemented with 1000 ng/inL Aug II at day 4; and iii) IVM/IVF and IVC in media without Ang II (Control). The blastocyst and hatching rates were recorded on days 6 to 8. Day 8 embryos were immunostained with primary and secondary antibodies against Na+/K+/ATPase subunits. Results: Addition of 1000 ng/mL Ang II during IVM and IVC was significantly increased the hatching rate of blastocysts on day 8 compared to the control. The total cell number was significantly increased by addition of Ang II to the IVM and IVC media, though the expression of Na+/K4/ATPase α1 and β1 subunits when IVC medium was supplemented with Ang II and β1 subunit when IVM medium was supplemented with Ang II were significantly increased compared to the control. Conclusion: In conclusion, this study for the first time demonstrated that addition of Ang II to the ovine IVM and IVC media could improve the hatching rate.


Related Articles

  • Physiological and pharmacological implications of AT1 versus AT2 receptors. Chung, Oliver; Kuhl, Hendrik; Stoll, Monika; Unger, Thomas // Kidney International Supplement;Sep1998, Issue 67, pS-95 

    Examines the implications of AT[sub 1] and AT[sub 2] receptors. Mediation on the effects of angiotensin II by AT[sub 1] receptors; Function of AT[sub 2] receptors in the tissues; Counteracting effects of AT[sub 1] and AT[sub 2] receptors on cellular growth and differentiation.

  • Angiotensin-II-derived reactive oxygen species on baroreflex sensitivity during hypertension: new perspectives. de Queiroz, Thyago M.; Monteiro, Matheus M. O.; Braga, Valdir A. // Frontiers in Physiology;May2013, Vol. 4, p1 

    Hypertension is a multifactorial disorder, which has been associated with the reduction in baroreflex sensitivity (BRS) and autonomic dysfunction. Several studies have revealed that increased reactive oxygen species (ROS) generated by nicotinamide adenine dinucleotide phosphate [NAD(P)H]...

  • Can angiotensin II be used for renoprotection? de Zeeuw, Dick // Kidney International;Mar2002, Vol. 61 Issue 3, p1176 

    Editorial. Investigates the potential of angiotensin II (Ang II) for renoprotection. Pathophysiological effects of the receptor-mediated actions of angiotensin II; Role in the mechanism underlying (patho)physiological effects; Role of renin-angiotensin-aldosterone system in the pathology of...

  • Two ACEs and a heart. Bernstein, Kenneth E. // Nature;6/20/2002, Vol. 417 Issue 6891, p799 

    Comments on an article by M.A. Crackower and colleagues on the cardiac function of angiotensin-converting enzyme (ACE) 2. Physiology of the peptide angiotensin II; Importance of angiotensin II; Discovery of ACE2.

  • AT-1 receptor antagonism modifies the mediation of endothelin-1, thromboxane A2, and catecholamines in the renal constrictor response to angiotensin II. Cediel, E.V.A.; de las Heras, Natalia; Sanz-Rosa, David; Velasco, Olga; Cachofeiro, Victoria; Lahera, Vicente // Kidney International;Jan2005 Supplement 93, Vol. 67, pS3 

    AT-1 receptor antagonism modifies the mediation of endothelin-1, thromboxane A 2, and catecholamines in the renal constrictor response to angiotensin II. Objective The present study investigated the consequences of partial AT 1 receptor blockade on the participation of catecholamines,...

  • What's my line?  // Psychology Today;Sep/Oct95, Vol. 28 Issue 5, p13 

    Reports on the identification of a protein called NRSF (neuron-restrictive silencer factor) that may be responsible for the formation of brain cells in a developing human embryo. Functions of the protein.

  • UK approves embryo 'gene editing'.  // Telegraph (Calcutta, India);Feb2016, p2 

    The article reports on the approval of gene editing in human embryos for research purposes in Great Britain.

  • Ghrelin: Central Nervous System Sites of Action in Regulation of Energy Balance. Fry, Mark; Ferguson, Alastair V. // International Journal of Peptides;2010, p1 

    Ghrelin, a peptide hormone secreted by the stomach, has been shown to regulate energy homeostasis by modulating electrical activity of neurons in the central nervous system (CNS). Like many circulating satiety signals, ghrelin is a peptide hormone and is unable to cross the blood-brain barrier...

  • Morphological analysis at compacting stage is a valuable prognostic tool for ICSI patients. Ebner, T.; Moser, M.; Shebl, O.; Sommergruber, M.; Gaiswinkler, U.; Tews, G. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jan2009, Vol. 18 Issue 1, p61 

    In terms of treatment outcome, little prognostic power is attributed to day-4 morphology. A day-4 score was applied to 56 patients separating non-compacting embryos from compacting (some areas of compaction) and fully compacted embryos. The latter were further subdivided according to the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics