TITLE

Effects of angiotensin II addition to the culture media on Na+/K+/ATPase expression and subsequent developmental competence of ovine embryos

AUTHOR(S)
Naderi, M. M.; Heidari, B.; Sarvari, A.; Borjian Boroujeni, S.; Behzadi, B.; Akhondi, M. M.; Shirazi, A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p19
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Presence of components of the renin-angiotensin system in mammalian ovaries and their involvement in ovarian physiology has been indicated. In the present study effects of angiotensin II (Ang II) addition to the maturation and embryo culture media on Na+/K+/ATPase expression and subsequent development of ovine embryos was evaluated. Materials and Methods: The abattoir-derived COCs (cumulus oocyte complexes) were randomly allocated into three experimental groups: I) IVM in medium supplemented with 1000 ng/mL Ang II; ii) IVM/IVF embryos followed by IVC wherein the resulting embryos were exposed to IVC medium supplemented with 1000 ng/inL Aug II at day 4; and iii) IVM/IVF and IVC in media without Ang II (Control). The blastocyst and hatching rates were recorded on days 6 to 8. Day 8 embryos were immunostained with primary and secondary antibodies against Na+/K+/ATPase subunits. Results: Addition of 1000 ng/mL Ang II during IVM and IVC was significantly increased the hatching rate of blastocysts on day 8 compared to the control. The total cell number was significantly increased by addition of Ang II to the IVM and IVC media, though the expression of Na+/K4/ATPase α1 and β1 subunits when IVC medium was supplemented with Ang II and β1 subunit when IVM medium was supplemented with Ang II were significantly increased compared to the control. Conclusion: In conclusion, this study for the first time demonstrated that addition of Ang II to the ovine IVM and IVC media could improve the hatching rate.
ACCESSION #
96841522

 

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