The relationship between sperm protamine deficiency and PRM1 and PRM2 genes changes in varicoceles

Talebi, A. R.; Heidari, M.; Tabibnejad, N.; Ghasemzadeh, J.; Nayeri, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p18
Academic Journal
Introduction: Varicocele is associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. It has been demonstrated that patients with varicocele have a sperm DNA fragmentation index and anomalies than healthy fertile men. Protamines play an important role in DNA packaging within the sperm nucleus. In order to evaluate the association of PRM1, PRM2 and protamine deficiency in sperm of varicocele patients, this study was conducted. Materials and Methods: In a case control study, 110 infertile men patients with varicocele and 105 controls were recruited. PRM1 and PRM2 gene mutations in extracted DNA samples, were assessed by PCR-denature polyacrylamide gel electrophoresis and then by DNA sequencing. The relationship between gene variations and semen parameters/ protamine deficiency was evaluated. Results: The results showed that sperm count, total motility (grades "a" and "b" and "c"), progressive motility (grades "a" and "b"), non-progressive motility and sperm morphology were significantly different between control group without gene mutation and varicocele group who had different gene variations (p<0.05). With regard to CM A3 test, the protamine deficiency was significantly higher in varicocele patients than controls (p<0.05). Conclusion: our results showed that PRM1 and PRM2 variations in varicocele patients are related to the production of spermatozoa with poor quality and more protamine deficiency. The latter may consider as one of the possible mechanisms of infertility due to varicocele.


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