Investigating the rate of SNP of H2BFWT gene in an Iranian population with idiopathic spermatogenesis impairment

Rasti, M.; Sheikhha, M.; Kalantar, S. M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p18
Academic Journal
Introduction: Spermatogenesis is a complex process including spermatogonial stem cell proliferation, meiosis and spermatid differentiation. Genetic variation of those genes involved in this process may play an important role in impaired spermatogenesis. Histones are the major nuclear proteins in eukaryotic cell nuclei that are responsible for the nucleosome structure of chromatin. The H2B family member W, testis specific (H2BFWT) gene encodes a testis specific histone that plays a crucial role in reorganization and remodeling of chromatin and epigenetic regulation during spermatogenesis, suggesting that the gene may be involved in spermatogenesis impairment. Materials and Methods: To test the speculation, the allele frequency of one single-nucleotide polymorphism locus in this gene, -9C >T was investigated in 92 infertile patients with idiopathic azoospermia or oligozoospermia and 100 fertile men as controls using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. Results: As the results, the frequency of -9C>T (48.9% vs. 51.1%, p=0.470, OR=0.783, 95% CI=0.444-1.382) was similar between patients and those in controls; these frequencies were the same in allele as well, -9C >T for oligozoospermia or azoospermia. Conclusion: These results indicated that the polymorphism -9C >T in H2BFWT gene is not associated with male infertility with idiopathic azoospermia or oligozoospermia in our patients, suggesting that H2BFWT gene may not be contribute to susceptibility to spermatogenesis impairment in Iranian population.


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