YBX2 involved in down regulation of Protamin2 expression in testicular spermatids of azoospermic men

Moghbelinejad, S.; Najafipour, R.; Aleyasin, A.; Rezaeian, Z.; Fallahi, P.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p17
Academic Journal
Introduction: PRM1/PRM2 ratio is important in spermatogenesis and male fertility; on the other hand uncoupling of PRM1 and PRM2 expression involves in the infertility of males. But the mechanisms implicit in this condition remain unclear. In this regard, we compared PRM1/PRM2 mRNA ratio and mRNA contents of YBX2 and Jhdm2a genes as two expression regulatory factors of these genes. Material and Methods: After RNA extraction and cDNA synthesis, Real-time quantitative (RT) PCR was used to evaluate the expression of PRM1, PRM2, YBX2 and Jhdm2a genes in testicular biopsies of 50 nonobstructive azoospermic and 12 normal testis tissues. Results: The PRM1/PRM2 mRNA ratio was 0.21± 0.13 in azoospermic samples and -0.8±0.22 in fertile samples, which was a significant difference. The amount of PRM2 mRNA was significantly reduced in azoospermic patients. Significant under expression of YBX2 gene was seen in azoospermic men in comparison to controls (p<0.001), and mRNA content of this gene showed positive correlation with PRM mRNA ratio (R=0.6, p=0.007). No significant difference was seen in Jhdm2a expression ratio between studied groups (p=0.3); no correlation was found between Jhdm2a mRNA content and the PRM mRNA ratio (R=0.2, p=0.3). Conclusion: Our results suggested that down regulation of YBX2 gene may be involved in PRM2 under expression, and this molecule can be a useful biomarker for predicting male infertility.


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