Morphological and ultrastructural studies of human spermatogonial stem cells from patients with maturation arrest

Mirzapour, T.; Tengku Ibrahim, T.; Movahedin, M.; Nowroozi, M.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p16
Academic Journal
Introduction: Destruction of spermatogonial stem cells (SSCs) along the cancer cells is one of the side effects of cancer treatments that can produce infertile patients. To preserve fertility in these patients, one hypothesis was that testicular biopsies are obtained prior to the treatment, and SSCs are isolated and preserved by cryopreservation and finally transplanted back into the patient's testis in a suitable time. Since small testicular biopsies do not contain sufficient SSCs to fully repopulate the testis after transplantation, in vitro propagation of hSSCs will be necessary to obtain an adequate number of cells for successful transplantation. Materials and Methods: hSSCs were isolated from testis biopsies and permitted to amplify in number by self-renewal in vitro in the presence of LIF and bFGF in DMEM for 5 weeks. Various types of human spermatogonia in culture system was identified and compared with testis tissue using morphological criteria, at the ultrastructural level. Results: Although many differences in various types of spermatogonia were identified but approximately were not observed remarkably difference between spermatogonial cells in culture system and testis tissue. Electron and light microscopic studies of hSSCs colonies and also assessment of gene expression did not show differentiated SSCs in the culture system. Conclusion: The results also showed that two-three weeks after culture is probably suitable time for transplantation of SSCs in recipient testis because in this time apoptosis has not been started in colony cells so that the population of apoptotic cells is low in the culture system.


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