TITLE

Effect of oxidative stress on KDM5D expression in mature mouse testis

AUTHOR(S)
Mokhtari, P.; Gourabi, H.; Mohseni Meybodi, A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p16
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Male factors are important causes of infertility. Two major causative factors of male infertility are oxidative stress (OS) and genetic factors. OS damages the sperm plasma membrane, the genome integrity and expression profile of genes involved in spermatogenesis. KDM5D or SMCY is one of these genes which its alteration is associated with male infertility. In this study the expression profile of KDM5D gene was evaluated in testis tissues of infertile after OS induction. Materials and Methods: Oxidative stress in adult mice testis was induced by injection of the 1:10 concentration of tertiary-butyl hydro-peroxide (TBHP). Adult male Balb/c mice were randomly selected. Case group included treated mice by TBHP for 2 weeks and control group treated only by injection of dFFO. Induced ROS levels in testes tissue samples of all mice measured by flow-cytometry. Consequently the expression of KDM5D gene was quantitatively measured in samples of both groups by real-time PCR. Results: According to flow-cytometry results, an increase of oxidative stress in ROS treated mice in comparison to control group was observed. Moreover, the expression level of SMCY gene was significantly decreased in TBHP treated mice (p<0.01). Conclusion: Oxidative stress can have detrimental effects on testicular tissue and alters the expression of some genes which are involved in spermatogenesis.
ACCESSION #
96841515

 

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