TITLE

Performance of PCR method on Amelogenin gene in maternal blood for sex determination of embryos

AUTHOR(S)
Shirali, S.; Khodadi, E.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p16
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Sex determination can not only be used for forensics investigations but can also be useful in prenatal diagnosis of fetus x-linked diseases and diagnosis of fetal abnormalities. Recently, the molecular techniques has special place and are highly important to determine gender. PCR methods using sequences that are both in X and Y chromosomes, such as ZFY and ZFX, Steroid Sulfatase (STS), Amelogenin gene, or only are on the Y chromosome like: SRY, DYS14, DYS1. Techniques used for sampling of the fetus are divided into two groups including invasive methods such as, chorionic villus sampling (CVS), Amniocentesis Precutaneous umblical blood sampling (PUBS), Fetal tissue sampling (FTS) methods and noninvasive methods that includes the detection of cell-free fetal DNA in serum or plasma. Materials and Methods: Maternal blood samples were collected and DNA was extracted from blood samples using modified phenol-chloroform extraction procedure. The primers of the Amelogenin gene were designed to yield products that would be readily distinguishable by their sizes upon agarose gel electrophoresis. Results: All of amplified samples which had been shown a single 467-bp fragment were interpreted as female and the others involved both the 467 and 341 bp amplification products were interpreted as male. This method could detect accurately the sex of male and females embryos by 90%. Conclusion: This assay provides a rapid and sensitive method for gender determination. The success of the procedure can be monitored by the presence of the X-chromosome band. The method contains an internal control which detects PCR failure. Two important merits of this research are its feasibility and reliability. All of these processes can be carried out in a regular laboratory or at an outside workstation and results are available within ten hours. By using PCR method on Amelogenin gene favorable results for sex determination of the fetus in the first trimester of pregnancy were obtained.
ACCESSION #
96841514

 

Related Articles

  • Rapid trisomy diagnosis (21, 18, and 13) using flourescent PCR and short tandem repeats:... Findlay, Ian; Toth, Tamas; Matthews, Paul; Marton, Tamás; Quirke, Philip; Papp, Zoltán // Journal of Assisted Reproduction & Genetics;May98, Vol. 15 Issue 5, p266 

    Presents a study which was conducted to determine whether flourescent polymerase chain reaction (PCR) could be of use in a prenatal diagnostic setting. How prenatal diagnosis of fetal trisomies is usually performed; What this requires; What the quantitative nature of PCR allows; How the study...

  • Preimplantation genetic diagnosis of familial adenomatous polyposis. Davis, T.; Song, B.; Cram, D. S. // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Nov2006, Vol. 13 Issue 5, p707 

    Familial adenomatous polyposis is a severe autosomal dominant cancer predisposition syndrome. It is characterized by the development of hundreds to thousands of polyps in the gastrointestinal tract, primarily in the colon, at a mean age of 16 years. Without a colectomy, colon cancer is...

  • Application of PGD for single gene disorders. B., Ismailoglu; G., Ozgon; F., Fiorentino; S., Demir; H., Karagozoglu; S, Unal; G., Karlikaya; S., Kahraman // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Apr2008 Supplement 3, Vol. 16, pS-46 

    Introduction: Preimplantation genetic diagnosis (PGD) is an essential alternative to prenatal diagnosis for couples at risk of having an affected child because of single gene disorder. When prenatal diagnosis was made, if the result were an affected fetus, termination of pregnancy would be...

  • Granulocyte-Macrophage Colony-Stimulating Factor: Potential Regulator.  // Fertility Weekly;5/16/2005, p11 

    The article cites a study on Granulocyte-macrophage colony-stimulating factor (GM-CSF), conducted by Barry Behr and colleagues. The researchers conducted an experiment using preimplantation mouse embryos. The study was published in a 2005 issue of the Journal of Assisted Reproduction and...

  • Very early prenatal diagnosis on coelomic cells using quantitative fluorescent polymerase chain reaction. Jauniaux, Eric; Cirigliano, Vincenzo; Adinolfi, Matteo // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2003, Vol. 6 Issue 4, p494 

    Presents a study that assessed the possibility of using the quantitative fluorescent polymerase chain reaction assay performed on DNA extracted from cells present in the extra-embryonic coelom for the detection of aneuploidies in prenatal diagnosis. Materials and methods; Results of the study.

  • Development and Application of Real-Time Quantitative Polymerase Chain Reaction Technique Using SYBR Green I in the Diagnosis of Down Syndrome. Kamyab, Ahmad Reza; Shahbazi, Shirin; Dibajnia, Parvin; Nosaeid, Mina Hayt; Akbari, Mohammad Taghi; Rafiee, Amir Reza; Tamiz, Katayun Akhlagh; Mahdian, Reza // Yakhteh Medical Journal;Summer2010, Vol. 12 Issue 2, p249 

    Objective: Rapid diagnosis of Trisomy 21 Syndrome (Down Syndrome) patients using Real-Time quantitative Polymerase Chain Reaction (Real-Time qPCR) in order to establish a novel method for prenatal diagnosis in the future. Materials and Methods: A total of 5 ml of peripheral blood was obtained...

  • The application of amplified TSPY and amelogenin genes from maternal plasma as a non-invasive bovine fetal DNA diagnosis. Davoudi, Arash; Seighalani, Ramin; Aleyasin, Seyed Ahmad; Tarang, Alireza; Radjabi, Rouhollah; Tahmoressi, Farideh // EurAsian Journal of Biosciences;2011, Vol. 5, p119 

    Some studies showed analysis of fetal DNA in maternal plasma had been introduced as a new method for non-invasive prenatal diagnosis. Fetal sexing is possible at 8th week of pregnancy, using maternal blood sample testing. The aim was providing a rapid, reliable and non-invasive method for sexing...

  • SRY sequence in maternal plasma: Implications for non-invasive prenatal diagnosis: First report from India. D'Souza, Edna; Nair, Sona; Nadkarni, Anita; Ghosh, Kanjaksha; Colah, Roshan B. // Indian Journal of Human Genetics;Jan-Apr2012, Vol. 18 Issue 1, p87 

    AIM: The presence of circulatory cell-free fetal DNA in maternal plasma has found new applications in non-invasive risk-free prenatal diagnosis. MATERIALS AND METHODS: We made use of a size separation approach along with real time polymerase chain reaction (PCR) to evaluate the use of fetal DNA...

  • Sweeping changes coming regarding fetal RhD genotyping?  // Contemporary OB/GYN;Jun2005, Vol. 50 Issue 6, p15 

    Reports on the accuracy of a polymerase chain reaction-based assay of antenatal maternal scrum in determining fetal Rh status, according to a study published in France in June 2005. Number of pregnant women involved in the study; Information on cases in which the fetal RhD status was failed to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics