Investigation of expression pattern of stem cell markers of mice uterine tissue in the presence of steroid hormones

Davoudi, M.; Zavareh, S.; Paylakhi, S. H.; Ghorbanian, M. T.; Mohebbi, S. R.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p14
Academic Journal
Introduction: Fluctuating levels of circulating estrogen and progesterone orchestrate regeneration of uterine tissue. It has been postulated that stem cells are likely responsible for this remodelling. The aim of this study was to investigate the effect of estradiol and progesterone hormones on the Oct4 and Sox2 expression at the protein level of uterine tissue in the ovariectomized mice. Materials and Methods: Adult female NMRI mice were underwent ovariectomy operation. After two weeks, following 5 days of treatment with 17β-estradiol, progesterone and combination of estradiol & progesterone, uterus was removed and Immunohistochemical staining (IHC) of pluripotent markers (Oct4 and Sox2) were performed on the paraffin sections of middle region of uterus horn. Results: IHC staining revealed different expression locations of the Oct4 and Sox2 in the endometrium and myometrium of treated groups. Oct4 and Sox2 expression were seen innuclei of few number of cells in the estradiol-treated group, whereas the Oct4 and Sox2 overexpression were detected in the cytoplasm of a large number of cells (non- pluripotent cells) in the endometrium and myometrium of progesterone treated groups. Conclusion: The present study indicated that expression of the Oct4 and Sox2 at protein level in the uterine tissue were affected by steroid hormones.


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