TITLE

The effect of Eicosapentaenoic acid (omega-3) on the expression of PGR in cumulus cells on mice

AUTHOR(S)
Roshangar, L.; Rad, J. Soleimani; Valipour, B.; Aghadavood, M.; Nikzad, B.; Shafaei, H.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p14
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Despite the known benefits of omega-3 fatty acids on health, there is little knowledge of its effect on fertility. Omega-3 fatty acids can modify gene and protein expression through altering the function of transcription factors controlling gene expression. Cumulus expansion, is a critical process for normal oocyte development, ovulation, and fertilization, this event needs to the synthesis of Progesterone receptors. The aim of this study was to determine if Omega-3 can affect on the expression of PGR in Cumulus cells. Materials and Methods: 30 female mice were kept in standard condition. Female mice were super ovulated with 10 unit hMG and 48 hours later 10 unit HCG as IP injection, for the collection of cumulus- oocyte complexes (COCs) mice were killed 36 hours later by cervical dislocation and COCs were collected from 6-8 weeks old bulb/c female mice oviduct by flashing method. COCs were cultured in 20cc universal medium supplemented with 20 omega-3 and overlaid with mineral oil and incubated for 4-6 hrs at 5% CO2 and 37°C. Surrounding cumulus cells were removed mechanically by using 20 hyaluronidase. The cumulus cells was stored at- 80°C until real-time PCR. PCR was performed by using a master mix containing CYBR green. The expansion level of mRNA PGR was evaluated by real-time PCR. Results: The result showed that the level of expansion of PGR in experimental group comparing with control was significantly increased. Conclusion: The result indicates that omega-3 could improve Cumulus expansion through increasing the amount of expansion of PGR.
ACCESSION #
96841510

 

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