TITLE

Determining daz genes copy number in idiopathic non-obstructive azoospermic and oligospermic men

AUTHOR(S)
Alimardanian, L.; Ahani, A.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p14
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Three distinct Azoospermia Factor (AZF) regions on the long arm of the Y chromosome (AZFa, AZFb, and AZFc) are essential for normal spermatogenesis. Four copies of the DAZ genes within the AZFc region are 99.99% identical which have the highest frequency of deletion in men with azoospermia and oligozoospermia. The multicopy nature of DAZ has limited the understanding of its actual role in human spermatogenesis. The current study was designed to evaluate the role of DAZ deletions and duplications in spermatogenesis. Materials and Methods: Totally, 115 azoospermic, 41 oligozoospermic and 113 fertile healthy men as controls were recruited for this study. AZFc region deletions and DAZ copy number changes were analyzed using six STS markers sY1191, sY1161, sY1197, sY1291, sY1206, sY1201. The presence and absence of each DAZ gene was evaluated using two STS markers sY581, sY587 by PCR and RFLP. Also copy numbers were analyzed by comparison of number of DAZ genes to the autosomal DAZL by Quantitative PCR followed by fragment analysis. Results: The gr/gr deletions were seen in 6.9, 7.3 and 1.7% of azoospermic, oligozoospermic and fertile men, respectively. On the other hand, b2/b3, b2/b4 deletions were found only in azoospermic group with a frequency of 4.3, 1.7% in each deleted region. And whole AZFc region deletions were found in 0.86 and 0.88% of the azoospermic and fertile men, respectively. Results of fragment analysis showed that deletions of DAZ copies with 1:1 ratio (DAZ/DAZL) were 5.2, 7.3 and 0.88% in azoospermic, oligozoospermic men and controls, respectively. DAZ copies with 0:1 ratio was 26.08 and 0.8% in azoospermic and fertile men, respectively. DAZ copies duplications with 3:1 ratio was 7.8, 2.4 and 7.9% and with 4:1 ratio was 16.5, 17.01 and 4.4% in azoospermic, oligozoospermic men and controls, respectively. Conclusion: The results of RFLP comparing to the STS-PCRs were not analyzable, suggesting a specific Y haplo groups in Iranian population that differ to the previous known haplo groups. Decreasing of DAZ copy number was associated with oligozoospermia, while neither azoospermia nor oligozoospermia was associated with increasing of DAZ copies.
ACCESSION #
96841509

 

Related Articles

  • PTEN and Akt expression during growth of human ovarian follicles. Goto, Maki; Iwase, Akira; Ando, Hisao; Kurotsuchi, Shozo; Harata, Toko; Kikkawa, Fumitaka // Journal of Assisted Reproduction & Genetics;Nov2007, Vol. 24 Issue 11, p541 

    Purpose To assess the expression of PTEN and total and phosphorylated Akt in human ovarian follicles during follicular growth. Methods Immunohistochemistry of ovarian tissues and Western blotting and immunofluorescence of primary cultured luteinized granulosa cells for PTEN and Akt. Results...

  • Antioxidant and estrogen combination in induction ovulation of infertile women. Jahromi, A. Rasekh; Maalhagh, M.; Alipour, F.; Pad, N. // Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p7 

    Introduction: Many studies reported that antioxidants such as vitamin E, vitamin C help to scavenge the oxygen radicals throughout the female reproductive tract that might improve the infertility treatment. The aim of this research is adjusted a regimen that is a combination of antioxidant and...

  • Unilateral cryptorchidism appears to compromise paternity. Kuznar, Wayne // Urology Times;Feb98, Vol. 26 Issue 2, p22 

    Reports on the finding that compromised spermatogenesis is one possible explanation for decreased paternity among formerly unilateral cryptorchid men. Infertility rate of 10 percent among men who had surgical correction of an undescended testis a few months after birth to age 14; Higher...

  • Human genetics and disease: Mouse models of male infertility. Cooke, Howard J.; Saunders, Philippa T. K. // Nature Reviews Genetics;Oct2002, Vol. 3 Issue 10, p790 

    Spermatogenesis is a complex process that involves stem-cell renewal, genome reorganization and genome repackaging, and that culminates in the production of motile gametes. Problems at all stages of spermatogenesis contribute to human infertility, but few of them can be modelled in vitro or in...

  • Developmental regulation of the bcl-2 family during spermatogenesis: Insights into the sterility of bcl-w-/- male mice. Meehan, T; Loveland, K L; de Kretser, D; Cory, S; Print, C G // Cell Death & Differentiation;Mar2001, Vol. 8 Issue 3, p225 

    Expression of bcl-w, a close relative of bcl-2 is essential for male fertility in mice. Although the initial wave of spermatogenesis in bcl-w -I- mice proceeds normally until 3- 4 weeks of age, adults fail to produce sperm. To clarify why bcl-w is essential for adult but not juvenile...

  • Spermatogonial stem cell research results encouraging. Guttman, Cheryl // Urology Times;May2000, Vol. 28 Issue 5, p24 

    Reports that ongoing studies are providing researchers with an improved understanding of spermatogenesis that may lead to new approaches for male infertility management and gene therapy, according to David J. Cozzolino, a fellow in reproductive medicine and surgery. Research showing how...

  • Examination of Idiopathic Infertility in the Presence of Normal FSH Levels.  // Fertility Weekly;1/14/2002, p16 

    Evaluates the hypophyso-gonadal axis by measuring the intratesticular concentrations of several steroids in relation with the serum hormonal status by researchers in France. Analysis on the role of testosterone and follicle stimulating hormone; Measurement of the steroid levels in testicular...

  • Hormonal Therapy Restores Spermatogenesis in IHH Men.  // Fertility Weekly;8/9/2004, p8 

    The article focuses on treatment of idiopathic hypogonadotrophic hypogonadism (IHH) by hormonal treatment in case of older patients. Spermatogenesis in older men with IHH was restored by exogenous gonadotropins. Treatment process involves measurement of serum testosterone up to six months to...

  • Comparative expression analysis of Septin 14 in testes of infertile men with normal spermatogenesis and spermatogenic failure. Shafipour, Maryam; Sabbaghian, Marjan; Shahhoseini, Maryam; Gilani, Mohammad Ali Sadighi // Iranian Journal of Reproductive Medicine;Mar2014, Vol. 12 Issue 3, p205 

    Background: Septins are an evolutionary conserved group of GTP-binding and filament-forming proteins that have diverse cellular roles. An increasing body of data implicates the septin family in the pathogenesis of diverse states including cancers, neurodegeneration, and male infertility....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics