Effect of vitrification on expression level of DNA methyltransferase3b (Dnmt3b) in mouse ovary

Koliforosh, M.; Shahhosseini, M.; Favaedi, R.; Ebrahimi, B.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p9
Academic Journal
Introduction: Cryopreservation of ovarian tissue is paramount for fertility preservation, with important clinical applications; especially for women suffering from an oncological condition. Vitrification has recently emerged as a new trend for biological specimen preservation. On the other hand, gene expression changes during vitrification can influence oocyte maturation and need to be studied. Methylation of mammalian DNA is a major epigenetic regulatory mechanism that plays a special role in gene expression regulation. Dnmt3b is responsible for de novo methylation and essential for genome stability, imprinting and embryonic development. The aim of the present study was to evaluate the effects of vitrification on mRNA expression level of DNA methyltransferase3b gene. Materials and Methods: Ovaries of 4-6-week old NMRI female mice were placed in two control and needle immersed (NIV) vitrification groups. In vitrification group, ovaries were transferred into equilibration and vitrification medium, and then immersed in liquid nitrogen after loading by acupuncture needle. Parallel to vitrification process, morphology of ovarian tissues in control and vitrification group were analyzed and compared by using hematoxylin & eosin staining. Then, the expression of Dnmtib gene was investigated by realtime PCR. Results: In morphological analysis, ovarian tissue integrity was well preserved in vitrification group and was similar to the control group. However, the result of this study showed that the expression level of Dnmtib gene in vitrification group was significantly higher in comparison with control group (p<0.05). Conclusion: Our finding can be considered as an evidence for differential expression pattern of genes in vitrified ovary tissues through cryopreservation process.


Related Articles

  • Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique. Rosendahl, Mikkel; Schmidt, Kirsten Tryde; Ernst, Erik; Emil Rasmussen, Per; Loft, Anne; Byskov, Anne Grethe; Andersen, Anders Nyboe; Andersen, Claus Yding // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Feb2011, Vol. 22 Issue 2, p162 

    This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n = 386) and autotransplantation of ovarian tissue (n = 18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological...

  • New kidneys for old. Diliner, Luisa // BMJ: British Medical Journal (International Edition);8/3/91, Vol. 303 Issue 6797, p267 

    Reports the challenges in the transplantation programs in Cambridge, England. Increase in the demand for organs; Criteria for brain stem death; Preservation of organs in cryobiology.

  • Fertility on Ice. Cook, Michael // Australasian Science;Sep2012, p48 

    The article focuses on the effects of cryopreservation and eventual transplantation of ovarian tissue on women.

  • Cryopreservation of ovarian tissue after pretreatment with a gonadotropin-releasing hormone agonist. Igarashi, Suguru; Suzuki, Nao; Osada, Marie; Takae, Seido; Tarumi, Wataru; Ishizuka, Bunpei // Reproductive Medicine & Biology;Dec2010, Vol. 9 Issue 4, p197 

    Purpose: The purpose of this study was to investigate whether slow-rate freezing or vitrification is better for cryopreservation of ovary tissues pretreated with gonadotropin-releasing hormone agonist. Methods: In this nonclinical study performed in rats, leuprorelin acetate was administered to...

  • Case Report of Subsequent Reimplantation and IVF Pregnancy.  // Fertility Weekly;10/3/2011, p9 

    The article presents a case study conducted in Israel on subsequent reimplantation and in vitro fertilization (IVF) pregnancy. The study suggests that cryopreservation and reimplantation of thin ovarian fragments can establish IVF pregnancy. Lead researcher A. Revel and colleagues conclude that...

  • Suspended Animation of Living Organisms.  // Trends Magazine;Apr2009, Issue 72, p24 

    The article discusses the theory of cryogenically freezing patients in a suspended animation of living organisms until future medical discoveries can revive them. However, the process of freezing or defrosting can severely damage vital cells. It notes that a new technology of slow freezing has...

  • Influence of Mesenchymal Stem Cells on Cryopreserved Tracheal Allografts in Rabbits. Hyunjo Kim // Korean Journal of Thoracic & Cardiovascular Surgery;2013, Vol. 46 Issue 5, p328 

    Background: Ischemic injury and the rejection process are the main reasons for graft failure in tracheal transplantation models. To enhance the acceptance, we investigated the influence of mesenchymal stem cells (MSCs) on tracheal allografts. Methods: Extracted tracheal grafts from New Zealand...

  • In Situ Reconstruction with Cryopreserved Arterial Allografts. Zhou, Wei; Lin, Peter H.; Bush, Ruth L.; Terramani, Thomas T.; Matsuura, John H.; Cox, Mitchell; Peden, Eric; Guerrero, Marlon; Silberfein, Eric J.; Dardik, Alan; Rosenthal, David; Lumsden, Alan B. // Texas Heart Institute Journal;2006, Vol. 33 Issue 1, p14 

    We designed this study to evaluate a multi-institutional experience regarding the efficacy of cryopreserved aortic allografts in the treatment of infected aortic prosthetic grafts or mycotic aneurysms. We reviewed clinical data of all patients from 4 institutions who underwent in situ aortic...

  • Human Umbilical Cord Blood Biology, Transplantation and Plasticity. Goldstein, Gal; Toren, Amos; Nagler, Arnon // Current Medicinal Chemistry;2006, Vol. 13 Issue 11, p1249 

    As the significance of hematopoietic stem cell transplantation (HSCT) is constantly rising, the scarcity of matched donors is proving to be a troubling issue. Cord blood (CB) is an important source of stem cells (SC) for transplantation. It has been used in the last two decades for approximately...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics