TITLE

Effect of vitrification on expression level of DNA methyltransferase3b (Dnmt3b) in mouse ovary

AUTHOR(S)
Koliforosh, M.; Shahhosseini, M.; Favaedi, R.; Ebrahimi, B.
PUB. DATE
June 2014
SOURCE
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p9
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Introduction: Cryopreservation of ovarian tissue is paramount for fertility preservation, with important clinical applications; especially for women suffering from an oncological condition. Vitrification has recently emerged as a new trend for biological specimen preservation. On the other hand, gene expression changes during vitrification can influence oocyte maturation and need to be studied. Methylation of mammalian DNA is a major epigenetic regulatory mechanism that plays a special role in gene expression regulation. Dnmt3b is responsible for de novo methylation and essential for genome stability, imprinting and embryonic development. The aim of the present study was to evaluate the effects of vitrification on mRNA expression level of DNA methyltransferase3b gene. Materials and Methods: Ovaries of 4-6-week old NMRI female mice were placed in two control and needle immersed (NIV) vitrification groups. In vitrification group, ovaries were transferred into equilibration and vitrification medium, and then immersed in liquid nitrogen after loading by acupuncture needle. Parallel to vitrification process, morphology of ovarian tissues in control and vitrification group were analyzed and compared by using hematoxylin & eosin staining. Then, the expression of Dnmtib gene was investigated by realtime PCR. Results: In morphological analysis, ovarian tissue integrity was well preserved in vitrification group and was similar to the control group. However, the result of this study showed that the expression level of Dnmtib gene in vitrification group was significantly higher in comparison with control group (p<0.05). Conclusion: Our finding can be considered as an evidence for differential expression pattern of genes in vitrified ovary tissues through cryopreservation process.
ACCESSION #
96841496

 

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