Oestrous cycle restoration after rat ovarian tissue autotransplantation, using hyaluronic acid hydrogel scaffold

Tavana, S.; Rezazadeh Valojerdi, M.; Azarnia, M.; Shahverdi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p3
Academic Journal
Introduction: One of the new emerging techniques to preserve reproductive potential of cancer patients is transplantation of ovarian tissue. Ischemia after transplantation leads to substantial follicular loss. So, we used hyaluronic acid (HA) hydrogel scaffold as a pro-angiogenic tool for restoration of cyclical ovarian function after ovarian tissue transplantation in a rat model. Materials and Methods: In this study, a total of 20 Wistar female rats weighing between 90 and 100 g were used. All these animals had normal estrous cycles according to vaginal cytology and were randomly divided into 2 groups for ovarian tissue graft surgery in diestrus stage: The ovarian tissues treated with HA hydrogel were designated as an experimental group (B) and without this scaffold were defined as control group (A). All grafts were transplanted into latissimus dorsi muscle. From the fourth day of post-surgery, ovarian function was monitored by taking vaginal smears and continued till restoration of first cycle. Results: Daily vaginal smear showed that the first estrous cycle began on the average of 15.9 and 13.4 days after transplantation in group A and group B respectively. Also resumption of ovarian function was happened in 11 -26 days after graft whereas in experimental group it was seen in 10-18 days after surgery. However no significant (p<0.5) differences were observed between the two groups. Conclusion: The use of HA hydrogel scaffold improves ovarian function and resumption of cyclic ovarian activity after transplantation but in our study this improvement was not impressive. Perhaps HA alone cannot overcome ischemic issue.


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