Evaluation of progesterone receptor α and β expression in endometrium of women with repeated implantation failure

Alaee, S.; Yeganeh, F.; Ghaffari Novin, M.; Allahveisi, A.
June 2014
Iranian Journal of Reproductive Medicine;Jun2014 Supplement, Vol. 12, p2
Academic Journal
Introduction: One of the most important limiting factors in ART (Assisted Reproductive Technologies), is repeated implantation failure (RIF). In the process of embryo implantation and establishment of a successful pregnancy, the accurate function of progesterone hormone is essential for creating a receptive endometrium. Progesterone hormone should bind to its receptors, PRα and PRβ in endometrial tissue to activate the transcription of genes essential for embryo implantation. Therefore, any noticeable alteration in the level of PRα and PRβ expression in endometrium results in attenuated response of endometrium to this hormone and can be related to a non-receptive endometrium. In this study we investigated possible in vivo role of altered PRα and PRβ expression in repeated implantation failure. Materials and Methods: Endometrial biopsy was performed for ten RIF's women and ten normal women in the midsecretory phase. Gene-specific primer pairs and Tagman probe for PRα, PRβ and GAPDH, as internal control, were designed using the beacon designer software 7. Then total RNA was extracted, single-stranded cDNA was synthesized and detection of target gene expression was performed using Real time PCR technique. Finally for or each sample the 2ACt was calculated and the results were indicated as relative expression. Results: The PRα and PRβ mRNA expression was significantly lower in the endometrium of RIF women compared to normal women. Conclusion: In conclusion, a lower level of PRα and PRP mRNA expression in endometrium of RIF women may cause alteration in function of progesterone hormone, leading to diminished endometrial receptivity and embryo implantation failure.


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