TITLE

Study of Effect of Mouse Embryos Lower Limb-Buds Co-Culture System on Differentiation of Mesenchymal Stem Cell to Neurons

AUTHOR(S)
Porlarimi, F. Z.; Khan Babaei, R.; Naji, T.; Porlarimi, H.
PUB. DATE
June 2013
SOURCE
Cell Journal (Yakhteh);Summer 2013 Supplement 1, Vol. 15 Issue Sup 1, p67
SOURCE TYPE
Academic Journal
DOC. TYPE
Abstract
ABSTRACT
Objective: One kind of adult stem cell that are most important and attracted the attention of most researchers is mesenchymal stem cell. Mesenchymal stem cell can differentiate in vitro into various kinds of mesenchymal cells such as cartilage, fat, muscle and non-mesenchymal cells including neural cells. In this study lower limb-bud tails and foots of mouse embryos co-culture have been used for differentian of mesenchymal stem cell into nerve cells. Materials and Methods: Mice Balb/C were killed, then bone marrow of femur bone were removed and cultured in culture flask containing DMEM/f12 subelement with %15fBS and penicillin-streptomycin antibiotic. The medium was changed once every 48 hours, after confluency, have been passage. After the third and fourth passage, mesenchymal stem cells co-cultured with lower limb-bud(tail and feet) respectively. One week later, the morphology of cells evaluated by kerziel viole staining for confirming the differentiation of Msc to neurons. We used flowcytometery for assessment of specific marker neural stem cells. Results: The result of this study showed that after two weeks of primary culture, Fusi Form like-fibroblast cells was observed with 70-75 percent confluency. Staining showed, morphologically modifications observed after differentiation were similar to morphologically modifications that occurs in neurons cells. Flowcytometry analysis showed that cells after induction will express neuronal marker Nestin and ß-tubulin. Conclusion: In this study of lower limb buds co-culture system, tail and feet for differentiation of mesenchymal stem cells into neuron cell are used. Result showed that Feeder layer cells within secretion of growth factor, FGF, EGF, RA cause is differentian of mesenchymal stem cell into neurons. These data confirm that in vitro hMScs can differentian to neurons cells.
ACCESSION #
93362957

 

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