Comparison of the effect of soluble TRAIL from progression-elevated gene-3 promoter on EC and ES cells

Jiang, X.; Xia, H.; Du, L.; Yang, S.; Lu, G.
May 2008
Reproductive BioMedicine Online (Reproductive Healthcare Limited;May2008 Supplement 2, Vol. 16 Issue S2, pS-35
Academic Journal
Introduction: Pluripotent embryonal carcinoma (EC) cells derived from teratocarcinomas that arise from transformed germ cells are generally considered to be the malignant counterparts of human embryonic stem cells. Thus, EC cells are regarded as a mode for study of cancer stem cells. The soluble TRAIL (sTRAIL) driven by progression-elevated gene-3 promoter can induce the apoptosis of EC cells whereas its effect on embryonic stem (ES) cells still remains unknown. Materials/Methods: A vector expressing tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL amino acids 114-281) driven by progression-elevated gene- 3 promoter (PEG-prom) was constructed. The EC and ES cells were transfected with the AAV-PEG-sTRAIL vector respectively. The cell apoptosis were evaluated by FACS and the expression of mRNA level of DcR1, DcR2, DR4 and DR5 with real-time PCR. Results: EC cells demonstrated significant higher apoptosis ration than ES cells with the influence of sTRAIL. Moreover, EC cells showed about (>2-fold) increase in DR5 expression whereas expression no significant change was found in the expression of DcR1, DcR2 and DR4. However, all of these genes showed no significant change in ES cells. Conclusion: Our results indicate that sTRAIL plays specific role in EC cells rather than ES cells, which provides a potential method for gene therapy of cancer stem cells.


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